Adhesion molecules involved in macrophage responses to wallerian degeneration in the murine peripheral nervous system

When a peripheral nerve is damaged the severed axon undergoes Wallerian degeneration, The distal nerve is infiltrated by large numbers of monocyte-derived macrophages which participate in the phagocytosis of degenerating myelin. In other tissues, adhesion molecules play a crucial role in leukocyte recruitment during inflammation. Blood-borne cells enter damaged tissue by interacting with adhesion molecules expressed on activated endothelium. Having crossed the endothelium, leukocytes must adhere and migrate within the tissue. We investigated the adhesion molecules involved in both stages of the macrophage response to transection of one sciatic nerve of BALB/c mice. By injecting monoclonal antibodies in vivo, before and after peripheral nerve injury, we showed that intercellular adhesion molecule-1 (ICAM-1) and integrins alpha(4) beta(1) (VLA-4) and alpha(M) beta(2) (type 3 complement receptor) are unlikely to be involved in the transendothelial migration of monocytes responding to peripheral nerve degeneration. We also studied the adhesion of macrophages within the endoneurium, using an in vitro adhesion assay. Macrophages showed much greater levels of adhesion to cryostat sections of transected nerves than to control nerves. This increased adhesion was partially inhibited by antibodies to the beta 1-integrin chain, and more strongly inhibited by the extracellular matrix molecules fibronectin and collagen. Adhesion was unaffected by laminin-1 and by antibodies to other adhesion molecules, including alpha(4) beta(1)- and alpha(5) beta(1)-integrins. Thus we conclude that monocyte entry into a degenerating peripheral nerve is independent of alpha(L) beta(2)/alpha(M) beta(2)-ICAM-1 or alpha(4) beta(1)/VCAM-1 interactions, and that adhesion within the endoneurium is mediated in pari by a beta 1-integrin other than alpha(4) beta(1) or alpha(5) beta(1).