Extending Polyketide Structural Diversity by Using Engineered Carboxylase/Reductase Enzymes

被引：3
|
作者：
Kundert, Jana [1 ]
Gulder, Tobias A. M. [1 ]
机构：
[1] Tech Univ Munich, Dept Chem, Ctr Integrated Prot Sci Munich CIPSM, Biosyst Chem, D-85748 Garching, Germany
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2016年 / 55卷 / 03期
关键词：
crotonyl-CoA carboxylase; reductases; enzymes; metabolic engineering; polyketide biosynthesis; CROTONYL-COA CARBOXYLASE/REDUCTASE; ANTIMYCIN BIOSYNTHESIS; UNITS; ACIDS;
D O I：
10.1002/anie.201510402
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
For a change: Enoyl-CoA carboxylase/reductases (ECRs) catalyze the selective α-carboxylation of α,β-unsaturated CoA-thioesters. Structure-based engineering of the active-site binding pocket of ECRs enabled significant alteration of their catalytic activity towards larger substrates. This facilitates the incorporation of unusual extender units into polyketide backbones, thus providing a novel method for the directed structural diversification of polyketides. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：858 / 860
页数：3
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