Lipase-catalyzed Production of Solid Fat Containing Conjugated Linoleic Acid in Binary Models

Solid fats were esterified with solid phase of rice bran oil (S-RBO), palm stearin (PS), and conjugated linoleic acid (CLA) at 2 substrate mole ratios (S-RBO:PS:CLA of 1: 1 :2 and 1:3:4). The major fatty acids were palmitic, oleic, and CLA in 36 hr products. The solid fat content (SFC) of the 1: 1 :2 product was 12.8% while the SFC of 1:3:4 product was 45.1% at 20 degrees C. The SFCs after 20 degrees C reduced when the reaction time increased from 1 to 36 hr, Suggesting that the change of triacylglycerol species was augmented by extending reaction time.