Isolation and separation of individual long-chain acyl-coenzyme A esters

被引：4
|
作者：
Shrago, E [1 ]
Woldegiorgis, G
机构：
[1] Univ Wisconsin, Dept Med, Madison, WI 53706 USA
[2] Univ Wisconsin, Dept Nutr Sci, Madison, WI 53706 USA
[3] Oregon Hlth & Sci Univ, OGI Sch Sci & Engn, Dept Biochem & Mol Biol, Beaverton, OR 97006 USA
来源：
ANALYTICA CHIMICA ACTA | 2002年 / 465卷 / 1-2期
关键词：
acyl-Co A; extraction; separation; HPLC;
D O I：
10.1016/S0003-2670(01)01559-8
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The isolation of intact long-chain acyl-coenzyme A (acyl Co A) esters is best accomplished by extraction of frozen powdered tissue by the procedure of Mancha et al. [Anal. Biochem. 68 (1975) 600], and the separation of the esters by reverse phase HPLC. The concentration of individual esters identified in various tissues reflect the metabolic state of the animal as well as the influence of dietary fat. This review discusses a number of modifications as well as the basic procedure that have increased the sensitivity and permitted quantitative identification of long-chain acyl-Co A esters. Overall, the method described has been instrumental in the quantitative analysis of tissue acyl-Co A esters and the development of the concept of long-chain acyl-Co A esters as signaling molecules in cellular metabolism. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：427 / 431
页数：5
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