A microbial transformation using Bacillus subtilis B7-S to produce natural vanillin from ferulic acid

被引：72

作者：

Chen, Peng ^{[1
]}

Yan, Lei ^{[2
]}

Wu, Zhengrong ^{[1
]}

Li, Suyue ^{[3
]}

Bai, Zhongtian ^{[4
]}

Yan, Xiaojuan ^{[3
]}

Wang, Ningbo ^{[3
]}

Liang, Ning ^{[3
]}

Li, Hongyu ^{[1
]}

机构：

[1] Lanzhou Univ, Sch Pharm, Donggang West Rd 199, Lanzhou 730020, Peoples R China

[2] Heilongjiang Bayi Agr Univ, Coll Life Sci & Technol, Daqing 163319, Peoples R China

[3] Gansu Inst Business & Technol, Yannan Rd 449, Lanzhou 730010, Peoples R China

[4] Lanzhou Univ, Hosp 1, Donggang West Rd 1, Lanzhou 730020, Peoples R China

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

关键词：

NEWLY ISOLATED STRAIN; 4-VINYLGUAIACOL; BIOCONVERSION; ISOEUGENOL; METABOLISM;

D O I：

10.1038/srep20400

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Bacillus subtilis strain B7-S screened from18 strains is an aerobic, endospore-forming, model organism of Gram-positive bacteria which is capable to form vanillin during ferulic acid bioconversion. The bioconversion of ferulic acid to vanillin by Bacillus subtilis B7-S (B. subtilis B7-S) was investigated. Based on our results, the optimum bioconversion conditions for the production of vanillin by B. subtilis B7-S can be summarized as follows: temperature 35 degrees C; initial pH 9.0; inoculum volume 5%; ferulic acid concentration 0.6 g/L; volume of culture medium 20%; and shaking speed 200 r/min. Under these conditions, several repeated small-scale batch experiments showed that the maximum conversion efficiency was 63.30% after 3 h of bioconversion. The vanillin products were confirmed by spectral data achieved from UV-vis, inductively coupled plasma atomic emission spectroscope (ICP-AES) and Fourier transform infrared spectrometer (FT-IR) spectra. Scanning electron microscopy (SEM) and transmission electron spectroscopy (TEM) results confirmed that the cell surface of B. subtilis plays a role in the induction of ferulic acid tolerance. These results demonstrate that B. subtilis B7-S has the potential for use in vanillin production through bioconversion of ferulic acid.

引用

页数：10

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