High sensitivity of flow cytometry improves detection of occult leptomeningeal disease in acute lymphoblastic leukemia and lymphoblastic lymphoma

被引：31

作者：

Del Principe, Maria Ilaria ^{[1
]}

Buccisano, Francesco ^{[1
]}

Cefalo, Mariagiovanna ^{[1
]}

Maurillo, Luca ^{[1
]}

Di Caprio, Luigi ^{[1
]}

Di Piazza, Fabio ^{[1
]}

Sarlo, Chiara ^{[2
]}

Angelis, Gottardo De ^{[1
]}

Consalvo, Maria Irno ^{[1
]}

Fraboni, Daniela ^{[1
]}

De Santis, Giovanna ^{[1
]}

Ditto, Concetta ^{[1
]}

Postorino, Massimiliano ^{[1
]}

Sconocchia, Giuseppe ^{[3
]}

Del Poeta, Giovanni ^{[1
]}

Amadori, Sergio ^{[1
]}

Venditti, Adriano ^{[1
]}

机构：

[1] Univ Roma Tor Vergata, Dipartimento Biopatol & Prevenz, Cattedra Ematol, Rome, Italy

[2] Policlin Univ Campus Biomed, Rome, Italy

[3] CNR, Ist Farmacol Traslaz, Dipartimento Med, Rome, Italy

来源：

ANNALS OF HEMATOLOGY | 2014年 / 93卷 / 09期

关键词：

Acute lymphoblastic leukemia; Lymphoblastic lymphoma; Cerebrospinal fluid; Conventional cytology; Flow cytometry; Leptomeningeal disease; NERVOUS-SYSTEM INVOLVEMENT; DOSE-INTENSIVE REGIMEN; CEREBROSPINAL-FLUID; HYPER-CVAD; HIGH-RISK; CHILDREN; CNS; CELLS;

D O I：

10.1007/s00277-014-2080-6

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Conventional cytology (CC) of cerebrospinal fluid (CSF) fails to demonstrate malignant cells in up to 45 % of patients with acute lymphoblastic leukemia or lymphoblastic lymphoma (ALL/LL) in whom occult leptomeningeal disease is present. Flow cytometry (FCM) is considered more sensitive than CC, but clinical implications of CC negativity/CC positivity are not yet established. CSF samples from 38 adult patients with newly diagnosed ALL/LL were examined. Five (13 %) and nine (24 %) specimens were CC positive-FC positive (FCMpos/CCpos) and CC negative-FC positive (CCneg/FCMpos), respectively. The remaining 24 (63 %) samples were double negative (CCneg/FCMneg) (p = 0.001). CCneg/FCMpos patients showed a significantly shorter overall survival (OS) compared to CCneg/FCMneg ones. In multivariate analysis, the status of single FCM positivity was demonstrated to affect independently duration of OS (p = 0.005). In conclusion, FCM significantly improves detection of leptomeningeal occult localization in ALL/LL and appears to anticipate an adverse outcome. Further prospective studies on larger series are needed to confirm this preliminary observation.

引用

页码：1509 / 1513

页数：5

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