High sensitivity of flow cytometry improves detection of occult leptomeningeal disease in acute lymphoblastic leukemia and lymphoblastic lymphoma

被引：31
作者：
Del Principe, Maria Ilaria [1 ]
Buccisano, Francesco [1 ]
Cefalo, Mariagiovanna [1 ]
Maurillo, Luca [1 ]
Di Caprio, Luigi [1 ]
Di Piazza, Fabio [1 ]
Sarlo, Chiara [2 ]
Angelis, Gottardo De [1 ]
Consalvo, Maria Irno [1 ]
Fraboni, Daniela [1 ]
De Santis, Giovanna [1 ]
Ditto, Concetta [1 ]
Postorino, Massimiliano [1 ]
Sconocchia, Giuseppe [3 ]
Del Poeta, Giovanni [1 ]
Amadori, Sergio [1 ]
Venditti, Adriano [1 ]
机构：
[1] Univ Roma Tor Vergata, Dipartimento Biopatol & Prevenz, Cattedra Ematol, Rome, Italy
[2] Policlin Univ Campus Biomed, Rome, Italy
[3] CNR, Ist Farmacol Traslaz, Dipartimento Med, Rome, Italy
来源：
ANNALS OF HEMATOLOGY | 2014年 / 93卷 / 09期
关键词：
Acute lymphoblastic leukemia; Lymphoblastic lymphoma; Cerebrospinal fluid; Conventional cytology; Flow cytometry; Leptomeningeal disease; NERVOUS-SYSTEM INVOLVEMENT; DOSE-INTENSIVE REGIMEN; CEREBROSPINAL-FLUID; HYPER-CVAD; HIGH-RISK; CHILDREN; CNS; CELLS;
D O I：
10.1007/s00277-014-2080-6
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Conventional cytology (CC) of cerebrospinal fluid (CSF) fails to demonstrate malignant cells in up to 45 % of patients with acute lymphoblastic leukemia or lymphoblastic lymphoma (ALL/LL) in whom occult leptomeningeal disease is present. Flow cytometry (FCM) is considered more sensitive than CC, but clinical implications of CC negativity/CC positivity are not yet established. CSF samples from 38 adult patients with newly diagnosed ALL/LL were examined. Five (13 %) and nine (24 %) specimens were CC positive-FC positive (FCMpos/CCpos) and CC negative-FC positive (CCneg/FCMpos), respectively. The remaining 24 (63 %) samples were double negative (CCneg/FCMneg) (p = 0.001). CCneg/FCMpos patients showed a significantly shorter overall survival (OS) compared to CCneg/FCMneg ones. In multivariate analysis, the status of single FCM positivity was demonstrated to affect independently duration of OS (p = 0.005). In conclusion, FCM significantly improves detection of leptomeningeal occult localization in ALL/LL and appears to anticipate an adverse outcome. Further prospective studies on larger series are needed to confirm this preliminary observation.
引用
收藏
页码：1509 / 1513
页数：5
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