Interferon-gamma improves impaired dentinogenic and immunosuppressive functions of irreversible pulpitis-derived human dental pulp stem cells

被引：39

作者：

Sonoda, Soichiro ^{[1
,2
]}

Yamaza, Haruyoshi ^{[3
]}

Ma, Lan ^{[1
,3
,4
]}

Tanaka, Yosuke ^{[1
]}

Tomoda, Erika ^{[1
,3
]}

Aijima, Reona ^{[1
,5
]}

Nonaka, Kazuaki ^{[3
]}

Kukita, Toshio ^{[1
]}

Shi, Songtao ^{[6
]}

Nishimura, Fusanori ^{[2
]}

Yamaza, Takayoshi ^{[1
]}

机构：

[1] Kyushu Univ, Grad Sch Dent Sci, Dept Mol Cell Biol & Oral Anat, Fukuoka 812, Japan

[2] Kyushu Univ, Grad Sch Dent Sci, Dept Periodontol, Fukuoka 812, Japan

[3] Kyushu Univ, Dept Pediat Dent, Grad Sch Dent Sci, Fukuoka 812, Japan

[4] Sun Yat Sen Univ, Dept Pediat Dent, Guanghua Sch Stomatol, Hosp Stomatol, Guangzhou 510275, Guangdong, Peoples R China

[5] Saga Univ, Fac Med, Dept Histol & Neuroanat, Saga 840, Japan

[6] Univ Penn, Dept Anat & Cell Biol, Sch Dent Med, Philadelphia, PA 19104 USA

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

基金：

日本学术振兴会;

关键词：

HUMAN BONE-MARROW; NF-KAPPA-B; INHIBITS OSTEOGENIC DIFFERENTIATION; NECROSIS-FACTOR-ALPHA; IMMUNOMODULATORY PROPERTIES; TISSUE REGENERATION; IFN-GAMMA; TNF; PHENOTYPE; OSTEOPOROSIS;

D O I：

10.1038/srep19286

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Clinically, irreversible pulpitis is treated by the complete removal of pulp tissue followed by replacement with artificial materials. There is considered to be a high potential for autologous transplantation of human dental pulp stem cells (DPSCs) in endodontic treatment. The usefulness of DPSCs isolated from healthy teeth is limited. However, DPSCs isolated from diseased teeth with irreversible pulpitis (IP-DPSCs) are considered to be suitable for dentin/pulp regeneration. In this study, we examined the stem cell potency of IP-DPSCs. In comparison with healthy DPSCs, IP-DPSCs expressed lower colony-forming capacity, population-doubling rate, cell proliferation, multipotency, in vivo dentin regeneration, and immunosuppressive activity, suggesting that intact IP-DPSCs may be inadequate for dentin/pulp regeneration. Therefore, we attempted to improve the impaired in vivo dentin regeneration and in vitro immunosuppressive functions of IP-DPSCs to enable dentin/pulp regeneration. Interferon gamma (IFN-gamma) treatment enhanced in vivo dentin regeneration and in vitro T cell suppression of IP-DPSCs, whereas treatment with tumor necrosis factor alpha did not. Therefore, these findings suggest that IFN-gamma may be a feasible modulator to improve the functions of impaired IP-DPSCs, suggesting that autologous transplantation of IFN-gamma-accelerated IP-DPSCs might be a promising new therapeutic strategy for dentin/pulp tissue engineering in future endodontic treatment.

引用

页数：12

共 54 条

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