Amperometric tyrosinase biosensor based on polyacrylamide microgels

An amperometric enzyme sensor using tyrosinase (PPO) entrapped in polyacrylarnide microgels has been developed for determination of phenolic compounds. Polyacrylamide microgels were obtained by the concentrated emulsion polymerization method. The crosslinking of the polymer matrix optimum to retain the enzyme and to allow the diffusion of the compounds involved in the enzyme reaction has been studied (4.0%) as well as the influence on the response of analytical parameters such as pH, temperature, enzyme load and working potential. The useful lifetime of the biosensor was 27 days and it was useful to determine monophenolics compounds (e.g. cresol, chlorciphenol) and diphenolics compounds (e.g. catechol and doparnme) by amperometric measurements at -100 mV (versus SCE) in a batch system. The results showed that the substrate structures have a great influence on the sensor response. (c) 2006 Elsevier B.V. All rights reserved.