Identification and expression analysis of IRAK-4 cDNA and its gene from the ayu Plecoglossus altivelis altivelis

被引：3
|
作者：
Suzuki, Kyuma [1 ]
Izumi, Shotaro [2 ]
Tanaka, Hideki [1 ]
Katagiri, Takayuki [3 ]
机构：
[1] Gunma Prefectural Fisheries Expt Stn, Maebashi, Gunma 3710036, Japan
[2] Tokai Univ, Course Appl Biol Sci, Dept Fisheries, Sch Marine Sci & Technol, Shimizu, Shizuoka 4248610, Japan
[3] Tokyo Univ Marine Sci & Technol, Grad Sch Marine Sci & Technol, Tokyo 1088477, Japan
来源：
FISHERIES SCIENCE | 2016年 / 82卷 / 01期
关键词：
Ayu Plecoglossus altivelis altivelis; Innate and adaptive immunities; Interleukin-1 receptor-associated kinase 4 (IRAK-4); mRNA expression analysis; Flavobacterium psychrophilum; MOLECULAR CHARACTERIZATION; ONCORHYNCHUS-MYKISS; RESPIRATORY BURST; INNATE; INTERLEUKIN-1; EVOLUTION; CLONING; PROTEIN; FISH; NEUTROPHILS;
D O I：
10.1007/s12562-015-0942-z
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
Interleukin-1 receptor-associated kinase 4 (IRAK-4) is an important component of the mammalian innate and adaptive defense system against infectious disease. In this study, ayu Plecoglossus altivelis altivelis IRAK-4 cDNA and genomic DNA were cloned and analyzed. The open reading frame of ayu IRAK-4 cDNA encodes 463 amino acids, including a death domain and a serine/threonine protein kinase domain of the mammalian IRAK-4 motif. The ayu IRAK-4 gene comprises 11 exons, and was found to be very similar to vertebrate IRAK-4 genes described thus far. Ayu IRAK-4 mRNA was expressed in all tissues, and predominantly in the gill, heart, intestine, whole kidney, and spleen. At 12 h after immersion challenge with Flavobacterium psychrophilum, ayu IRAK-4 mRNA expression in the whole blood was up-regulated (21.6-fold) compared to the control. The present study suggests that ayu IRAK-4 may be involved in the immune response.
引用
收藏
页码：47 / 57
页数：11
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