Expression and modification of PKA and AKAPs during meiosis in rat oocytes

被引：22
作者：
Kovo, M
Schillace, RV
Galiani, D
Josefsberg, LB
Carr, DW
Dekel, N [1 ]
机构：
[1] Weizmann Inst Sci, Dept Regulat Biol, IL-76100 Rehovot, Israel
[2] Vet Affairs Med Ctr, Dept Med, Portland, OR 97201 USA
[3] Oregon Hlth & Sci Univ, Portland, OR 97201 USA
来源：
MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2002年 / 192卷 / 1-2期
关键词：
PKA; AKAP; meiosis; oocyte;
D O I：
10.1016/S0303-7207(02)00084-9
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Meiosis in oocytes is initiated during fetal life, arrested around birth and resumed after puberty. Meiotic arrest is controlled by a cAMP-dependent protein kinase (PKA)-mediated cAMP action. We examined oocytes for the presence and modulation of the regulatory (R) subunits of PKA and the A-kinase anchoring proteins (AKAPs) that target PKA to specific subcellular locations. We found that rat oocytes express the two regulatory subunit isoforms, RI and RII of PKA. Immunocytochemistry revealed that the regulatory subunits underwent cellular translocation upon resumption of meiosis. We also demonstrated the presence of a novel 140 kDa AKAP, AKAP140 that exhibited a retarded electrophoretic motility at reinitiation of meiosis. The mobility shift of AKAP140 was susceptible to alkaline phosphatase and prevented by inhibition of p34cdc2 kinase. We conclude that rat oocytes express AKAP140 that is phosphorylated during meiosis. AKAP140 phosphorylation is sensitive to p34cdc2 kinase inhibitors. We hypothesize that AKAP140 and its phosphorylation state may influence the translocation of the R subunits of PKA throughout resumption of meiosis. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
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收藏
页码：105 / 113
页数：9
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