Molecular characterization of Pleurotus eryngii varieties occurring in Italy

被引：0

作者：

Villani, Alessandra ^{[1
]}

Galli, Emanuela ^{[2
]}

Paciolla, Costantino ^{[3
]}

Stea, Gaetano ^{[1
]}

Logrieco, Antonio Francesco ^{[1
]}

Siniscalco, Carmine ^{[4
]}

Mule, Giuseppina ^{[1
]}

Susca, Antonia ^{[1
]}

机构：

[1] CNR, Inst Sci Food Prod, I-70126 Bari, Italy

[2] CNR, Inst Agroenvironm & Forest Biol, I-00015 Rome, Italy

[3] Univ Bari, Dept Biol, I-70125 Bari, Italy

[4] Natl Inst Environm Protect & Res ISPRA, I-00144 Rome, Italy

来源：

SYDOWIA | 2015年 / 67卷

关键词：

ef1-a; Pleurotus eryngii species complex; SNPs; rpb2; molecular phylogeny; PHYLOGENETIC-RELATIONSHIPS; SEQUENCE-ANALYSIS; COMPLEX; IDENTIFICATION; DIVERSITY; MUSHROOMS; TAXA; RPB2; NOV; DNA;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Pleurotus eryngii species complex is an economically important group which includes several closely related varieties, whose genetic discrimination is still not clear. One hundred and ten Italian strains of Pleurotus eryngii belonging to the varieties elaeoselini, eryngii, ferulae and thapsiae and P. nebrodensis were analysed by sequencing two housekeeping genes (ef1-a and rpb2), in order to find molecular markers for the identification of different varieties. Sequence analysis of partial ef1-a and rpb2 genes, allowed identification of some conserved nucleotide positions within each variety but variable among var. elaeoselini, var. eryngii, var. ferulae, var. thapsiae and P. nebrodensis, allowing their discrimination. Phylogenetic analysis from the data of the two genes data set showed that var. elaeoselini, var. thapsiae, var. ferulae and var. eryngii are closely related to each other, and confirm P. nebrodensis as a separate clade.

引用

页码：33 / 43

页数：11

共 35 条

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