Molecular cloning and expression analysis of a novel member of the Disintegrin and Metalloprotease-Domain (ADAM) family

被引：13

作者：

Brachvogel, B

Reichenberg, D

Beyer, S

Jehn, B

von der Mark, K

Bielke, W

机构：

[1] Univ Erlangen Nurnberg, Dept Expt Med 1, D-91054 Erlangen, Germany

[2] Univ Erlangen Nurnberg, Dept Internal Med 3, Div Mol Immunol, D-91054 Erlangen, Germany

来源：

GENE | 2002年 / 288卷 / 1-2期

关键词：

Disintegrin-Metalloprotease; testase; development; testis; sequence; in situ; fertilization; sperm;

D O I：

10.1016/S0378-1119(02)00508-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We cloned and characterized the cDNA and the expression pattern of a novel member of the murine 'Disintegrin and Metalloprotease-Domain Family' (ADAM). The predicted protein sequence reveals highest homology to the testase-subgroup, composed of ADAM 24, ADAM 25 and ADAM 26 and is therefore called testase 4. Reverse transcription-polymerase chain reaction showed a strong expression of testase 4 in the adult testis, but not in any other organ or embryonic stage tested. Careful characterization by in situ hybridization confirmed specific expression of testase 4 in maturing sperm cells of 6-week-old mice, whereas no specific hybridization pattern was detectable in testes of 2.5-week-old mice. These data indicate a correlation between testase 4 expression and spermatogenesis and/or fertilization. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：203 / 210

页数：8

共 43 条

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