Molecular cloning and expression analysis of a novel member of the Disintegrin and Metalloprotease-Domain (ADAM) family

被引：13
作者：
Brachvogel, B
Reichenberg, D
Beyer, S
Jehn, B
von der Mark, K
Bielke, W
机构：
[1] Univ Erlangen Nurnberg, Dept Expt Med 1, D-91054 Erlangen, Germany
[2] Univ Erlangen Nurnberg, Dept Internal Med 3, Div Mol Immunol, D-91054 Erlangen, Germany
来源：
GENE | 2002年 / 288卷 / 1-2期
关键词：
Disintegrin-Metalloprotease; testase; development; testis; sequence; in situ; fertilization; sperm;
D O I：
10.1016/S0378-1119(02)00508-5
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
We cloned and characterized the cDNA and the expression pattern of a novel member of the murine 'Disintegrin and Metalloprotease-Domain Family' (ADAM). The predicted protein sequence reveals highest homology to the testase-subgroup, composed of ADAM 24, ADAM 25 and ADAM 26 and is therefore called testase 4. Reverse transcription-polymerase chain reaction showed a strong expression of testase 4 in the adult testis, but not in any other organ or embryonic stage tested. Careful characterization by in situ hybridization confirmed specific expression of testase 4 in maturing sperm cells of 6-week-old mice, whereas no specific hybridization pattern was detectable in testes of 2.5-week-old mice. These data indicate a correlation between testase 4 expression and spermatogenesis and/or fertilization. (C) 2002 Elsevier Science B.V. All rights reserved.
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收藏
页码：203 / 210
页数：8
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