MacA a diheme c-type cytochrorne involved in Fe(III) reduction by Geobacter sulfurreducens

被引:87
作者
Butler, JE [1 ]
Kaufmann, F [1 ]
Coppi, MV [1 ]
Núñez, C [1 ]
Lovley, DR [1 ]
机构
[1] Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA
关键词
D O I
10.1128/JB.186.12.4042-4045.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A 36-kDa diheme c-type cytochrome abundant in Fe(III)-respiring Geobacter sulfurreducens, designated MacA, was more highly expressed during growth with Fe(III) as the electron acceptor than with fumarate. Although MacA has homology to proteins with in vitro peroxidase activity, deletion of macA had no impact on response to oxidative stress. However, the capacity for Fe(III) reduction was greatly diminished, indicating that MacA, which is predicted to be localized in the periplasm, is a key intermediate in electron transfer to Fe(III).
引用
收藏
页码:4042 / 4045
页数:4
相关论文
共 44 条
[1]   Purification and characterization of triheme cytochrome c7 from the metal-reducing bacterium, Geobacter metallireducens [J].
Afkar, E ;
Fukumori, Y .
FEMS MICROBIOLOGY LETTERS, 1999, 175 (02) :205-210
[2]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[3]   Stimulating the in situ activity of Geobacter species to remove uranium from the groundwater of a uranium-contaminated aquifer [J].
Anderson, RT ;
Vrionis, HA ;
Ortiz-Bernad, I ;
Resch, CT ;
Long, PE ;
Dayvault, R ;
Karp, K ;
Marutzky, S ;
Metzler, DR ;
Peacock, A ;
White, DC ;
Lowe, M ;
Lovley, DR .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (10) :5884-5891
[4]   A NEW-GENERATION OF INFORMATION-RETRIEVAL TOOLS FOR BIOLOGISTS - THE EXAMPLE OF THE EXPASY WWW SERVER [J].
APPEL, RD ;
BAIROCH, A ;
HOCHSTRASSER, DF .
TRENDS IN BIOCHEMICAL SCIENCES, 1994, 19 (06) :258-260
[5]  
AUSUBEL FM, 1999, CURRENT PROTOCOLS MO, V2
[6]   GEOBACTER SULFURREDUCENS SP-NOV, A HYDROGEN-OXIDIZING AND ACETATE-OXIDIZING DISSIMILATORY METAL-REDUCING MICROORGANISM [J].
CACCAVO, F ;
LONERGAN, DJ ;
LOVLEY, DR ;
DAVIS, M ;
STOLZ, JF ;
MCINERNEY, MJ .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (10) :3752-3759
[7]   Geobacter metallireducens accesses insoluble Fe(III) oxide by chemotaxis [J].
Childers, SE ;
Ciufo, S ;
Lovley, DR .
NATURE, 2002, 416 (6882) :767-769
[8]   Role of accurate mass measurement (±10 ppm) in protein identification strategies employing MS or MS MS and database searching [J].
Clauser, KR ;
Baker, P ;
Burlingame, AL .
ANALYTICAL CHEMISTRY, 1999, 71 (14) :2871-2882
[9]   Development of a genetic system for Geobacter sulfurreducens [J].
Coppi, MV ;
Leang, C ;
Sandler, SJ ;
Lovley, DR .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (07) :3180-3187
[10]   Cloning, overproduction and characterization of cytochrome c peroxidase from the purple phototrophic bacterium Rhodobacter capsulatus [J].
De Smet, L ;
Pettigrew, GW ;
Van Beeumen, JJ .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 2001, 268 (24) :6559-6568