Defensin from the ornate sheep tick Dermacentor marginatus and its effect on Lyme borreliosis spirochetes

被引：20

作者：

Chrudimska, Tereza ^{[1
,2
]}

Cerovsky, Vaclav ^{[3
]}

Slaninova, Jirina ^{[3
]}

Rego, Ryan O. M. ^{[1
]}

Grubhoffer, Libor ^{[1
,2
]}

机构：

[1] Acad Sci Czech Republ, Inst Parasitol, Ctr Biol, CR-37005 Ceske Budejovice, Czech Republic

[2] Univ South Bohemia, Fac Sci, Ceske Budejovice 37005, Czech Republic

[3] Acad Sci Czech Republ, Inst Organ Chem & Biochem, CR-16610 Prague 6, Czech Republic

来源：

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2014年 / 46卷 / 02期

关键词：

Tick; Dermacentor marginatus; Defensin; Borrelia afzelii; Antimicrobial activity; Peptide synthesis; AMERICAN DOG TICK; IXODES-SCAPULARIS; ANTIMICROBIAL PEPTIDES; VARIABILIS ACARI; HARD TICK; IXODIDAE; IDENTIFICATION;

D O I：

10.1016/j.dci.2014.04.005

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Expression of the previously reported defensin of the tick Dermacentor marginatus (defDM) was analysed in different organs by RT-PCR. mRNA of the defDM gene was detected in the hemolymph, midgut and salivary glands. Moreover defDM was isolated from the tick hemolymph using RP-HPLC and its sequence was determined by mass spectrometry and Edman degradation. Synthetic peptide was used for determining biological activities. The results showed an anti-Gram-positive bacterial role for the defensin. As D. marginatus ticks appear not to be vectors of the Lyme disease agent of the complex Borrelia burgdorferi sensu lato, we tested the influence of defDM on Borrelia afzelii. There is a very clear borrelicidal activity of the defensin, which is concentration dependent and suggests a possible role in the clearing of Borrelia ingested by D. marginatus ticks. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：165 / 170

页数：6

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