Determination of melamine impurities by capillary zone electrophoresis with UVand quadrupole time-of-flight mass spectrometric detection

被引:9
作者
Himmelsbach, Markus [1 ]
Thuy Diep Thanh Vo [2 ]
机构
[1] Johannes Kepler Univ Linz, Inst Analyt Chem, A-4040 Linz, Austria
[2] Borealis Polyolefine GmbH, Linz, Austria
关键词
CZE; Melamine; Melamine impurities; quadrupole TOF MS; ELECTROSPRAY-IONIZATION; LIQUID-CHROMATOGRAPHY; ION-TRAP; RESINS; MILK; MS;
D O I
10.1002/elps.201300493
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A method based on CZE coupled to quadrupole TOF MS (Q-TOF MS), which is suitable to analyze seven common melamine impurities is presented. MS compatible formic acid based BGEs with various concentrations of organic modifiers were investigated using UV detection to determine the best separation system regarding resolution and speed. The optimized BGE consisted of 350 mM formic acid with 10% ACN and migration times were <6 min. The detection limits were considerably improved when ESI MS was applied instead of UV detection. In addition the instrument allows quantification with good linearity and repeatability in the relevant concentration range. The analytical characteristics of the method were evaluated for melamine, guanylurea, diaminotriazine, ureidomelamine, guanlymelamine, melam, methylmelamine, and dimethylmelamine both with UV and MS detection. Several different melamine samples were analyzed und the determined impurity levels were in the range from 0.02 to 2.3%.
引用
收藏
页码:1362 / 1367
页数:6
相关论文
共 16 条
  • [1] Review of analytical techniques used in proficiency-testing programs for melamine in animal feed and milk
    Chu, Patricia W. S.
    Chan, Kimmy M.
    Cheung, Samuel T. C.
    Wong, Yiu-chung
    [J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2010, 29 (09) : 1014 - 1026
  • [2] Analysis of melamine resins by capillary zone electrophoresis with electrospray ionization-mass spectrometric detection
    Cook, HA
    Klampfl, CW
    Buchberger, W
    [J]. ELECTROPHORESIS, 2005, 26 (7-8) : 1576 - 1583
  • [3] High performance liquid chromatography electrospray ionisation and atmospheric pressure chemical ionisation mass spectrometry for the analysis of butylated amino resins
    Favretto, D
    Traldi, P
    Marcelli, A
    [J]. EUROPEAN MASS SPECTROMETRY, 1998, 4 (05): : 371 - 378
  • [4] Analysis of methylated melamines in reaction mixtures by CZE-MS
    Himmelsbach, Markus
    Schmid, Thomas
    Buchberger, Wolfgang
    Schwarzinger, Clemens
    List, Manuela
    [J]. ELECTROPHORESIS, 2010, 31 (07) : 1194 - 1200
  • [5] Highly sensitive detection of melamine and its derivatives by capillary electrophoresis coupled with online preconcentration techniques
    Jin, Yue
    Meng, Lingchen
    Li, Meixian
    Zhu, Zhiwei
    [J]. ELECTROPHORESIS, 2010, 31 (23-24) : 3913 - 3920
  • [6] Analysis of melamine in milk powder by CZE using UV detection and hyphenation with ESI quadrupole/TOF MS detection
    Klampfl, Christian W.
    Andersen, Liisa
    Haunschmidt, Manuela
    Himmelsbach, Markus
    Buchberger, Wolfgang
    [J]. ELECTROPHORESIS, 2009, 30 (10) : 1743 - 1746
  • [7] CHARACTERIZATION OF METHOXYMETHYLMELAMINE RESINS BY LIQUID-CHROMATOGRAPHY AND MASS-SPECTROMETRY
    LONGORDO, E
    PAPAZIAN, LA
    CHANG, TL
    [J]. JOURNAL OF LIQUID CHROMATOGRAPHY, 1991, 14 (11): : 2043 - 2063
  • [8] Marcelli A, 1997, RAPID COMMUN MASS SP, V11, P1321
  • [9] Nielen MWF, 1996, RAPID COMMUN MASS SP, V10, P74
  • [10] Ripperger W., 2002, Casale Chemicals SA EP1188751 (A1), Patent No. 1188751