Evidence for a secretory pathway Ca2+-ATPase in sea urchin spermatozoa

被引：22

作者：

Gunaratne, Herath Jayantha ^{[1
]}

Vacquier, Victor D. ^{[1
]}

机构：

[1] Univ Calif San Diego, Scripps Inst Oceanog, Ctr Marine Biotechnol & Biomed, La Jolla, CA 92093 USA

来源：

FEBS LETTERS | 2006年 / 580卷 / 16期

关键词：

bisphenol; intracellular calcium; invertebrate fertilization; PMCA; SPCA;

D O I：

10.1016/j.febslet.2006.06.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plasma membrane, sarco-endoplasmic reticulum and secretory pathway Ca2+-ATPases (designated PMCA, SERCA and SPCA) regulate intracellular Ca2+ in animal cells. The presence of PMCA, and the absence of SERCA, in sea urchin sperm is known. By using inhibitors of Ca2+-ATPases, we now show the presence of SPCA and Ca2+ store in sea urchin sperm, which refills by SPCA-type pumps. Immunofluorescence shows SPCA localizes to the mitochondrion. Ca2+ measurements reveal that similar to 75% of Ca2+ extrusion is by Ca2+ ATPases and 25% by Na+ dependent Ca2+ exchanger/s. Bisphenol, a Ca2+ ATPase inhibitor, completely blocks the acrosome reaction, indicating the importance of Ca+-ATPases in fertilization. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3900 / 3904

页数：5

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