Fullerenol Quantum Dots-Based Highly Sensitive Fluorescence Aptasensor for Patulin in Apple Juice

被引：13

作者：

Pang, Hua ^{[1
,2
,3
]}

Li, Hui ^{[1
,2
,3
,4
,5
]}

Zhang, Wen ^{[1
,2
,3
,4
,5
]}

Mao, Jin ^{[1
,3
,4
,5
]}

Zhang, Liangxiao ^{[1
,3
,5
]}

Zhang, Zhaowei ^{[1
,3
,4
,5
]}

Zhang, Qi ^{[1
,2
,3
,4
,5
]}

Wang, Du ^{[1
,5
]}

Jiang, Jun ^{[1
,5
]}

Li, Peiwu ^{[1
,2
,3
,4
,5
]}

机构：

[1] Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430062, Peoples R China

[2] Minist Agr & Rural Affairs, Key Lab Biol & Genet Improvement Oil Crops, Wuhan 430062, Peoples R China

[3] Natl Reference Lab Agr Testing Biotoxin, Wuhan 430062, Peoples R China

[4] Minist Agr & Rural Affairs, Key Lab Detect Mycotoxins, Wuhan 430062, Peoples R China

[5] Minist Agr & Rural Affairs, Lab Qual & Safety Risk Assessment Oilseed Prod Wu, Wuhan 430062, Peoples R China

来源：

TOXINS | 2022年 / 14卷 / 04期

基金：

中国国家自然科学基金;

关键词：

patulin; fullerenol quantum dots; aptasensor; fluorescence; MYCOTOXIN PATULIN; PLATFORM; BINDING; POLYMER; CELLS;

D O I：

10.3390/toxins14040272

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A highly selective and sensitive aptasensor for detecting patulin (PAT) was constructed based on the fluorescence quenching of fullerenol quantum dots (FOQDs) towards carboxytetramethylrhodamine (TAMRA) through PET mechanism. The pi-pi stacking interaction between PAT aptamer and FOQDs closed the distance between TAMRA and FOQDs and the fluorescence of TAMRA was quenched with maximum quenching efficiency reaching 85%. There was no non-specific fluorescence quenching caused by FOQDs. In the presence of PAT, the PAT aptamer was inclined to bind with PAT and its conformation was changed. Resulting in the weak pi-pi stacking interaction between PAT aptamer and FOQDs. Therefore, the fluorescence of TAMRA recovered and was linearly correlated to the concentration of PAT in the range of 0.02-1 ng/mL with a detection limit of 0.01 ng/mL. This PAT aptasensor also performed well in apple juice with linear dynamic range from 0.05-1 ng/mL. The homogeneous fluorescence aptasensor shows broad application prospect in the detection of various food pollutants.

引用

页数：10

共 35 条

[1] Ultrasensitive Fluorescent miRNA Biosensor Based on a "Sandwich" Oligonucleotide Hybridization and Fluorescence Resonance Energy Transfer Process Using an Ln(III)-MOF and Ag Nanoparticles for Early Cancer Diagnosis: Application of Central Composite Design [J].