Novel Inactivated Subtype B Avian Metapneumovirus Vaccine Induced Humoral and Cellular Immune Responses

被引：11

作者：

Bao, Yuanling ^{[1
]}

Yu, Mengmeng ^{[1
]}

Liu, Peng ^{[1
]}

Hou, Fujun ^{[2
]}

Muhammad, Farooque ^{[1
]}

Wang, Zhihao ^{[1
]}

Li, Xinyi ^{[1
]}

Zhang, Zhuo ^{[1
]}

Wang, Suyan ^{[1
]}

Chen, Yuntong ^{[1
]}

Cui, Hongyu ^{[1
]}

Liu, Aijing ^{[1
]}

Qi, Xiaole ^{[1
]}

Pan, Qing ^{[1
]}

Zhang, Yanping ^{[1
]}

Gao, Li ^{[1
]}

Li, Kai ^{[1
]}

Liu, Changjun ^{[1
]}

He, Xijun ^{[1
]}

Wang, Xiaomei ^{[1
]}

Gao, Yulong ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, State Key Lab Vet Biotechnol, Avian Immunosuppress Dis Div, Harbin Vet Res Inst, Harbin 150069, Peoples R China

[2] Aohan Cty Breeding & Extens Ctr, Chifeng 024300, Inner Mongolia, Peoples R China

来源：

VACCINES | 2020年 / 8卷 / 04期

关键词：

avian metapneumovirus; inactivated vaccine; adjuvant; INFECTION; TURKEYS; PNEUMOVIRUS; PATHOGENICITY; CHICKENS; IMMUNOSUPPRESSION; EXPRESSION; ANTIBODIES; EFFICACY; SEQUENCE;

D O I：

10.3390/vaccines8040762

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Avian metapneumovirus (aMPV), a highly contagious agent, is widespread and causes acute upper respiratory tract disease in chickens and turkeys. However, currently, there is no vaccine licensed in China. Herein, we describe the development of an inactivated aMPV/B vaccine using the aMPV/B strain LN16. Combined with a novel adjuvant containing immune-stimulating complexes (ISCOMs), the novel vaccine could induce high virus-specific and VN antibodies. In addition, it activated B and T lymphocytes and promoted the expression of IL-4 and IFN-gamma. Importantly, boosting vaccination with the inactivated aMPV/B vaccine could provide 100% protection against aMPV/B infection with reduced virus shedding and turbinate inflammation. The protection efficacy could last for at least 6 months. This study yielded a novel inactivated aMPV/B vaccine that could serve as the first vaccine candidate in China, thus contributing to the control of aMPV/B and promoting the development of the poultry industry.

引用

页码：1 / 13

页数：13

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