Successive pretreatment and enzymatic saccharification of sugarcane bagasse in a packed bed flow-through column reactor aiming to support biorefineries

被引：32
作者：
Teran-Hilares, R. [1 ]
Resendiz, A. L. [2 ]
Martinez, R. T. [3 ]
Silva, S. S. [1 ]
Santos, J. C. [1 ]
机构：
[1] Univ Sao Paulo, Escola Engn Lorena, Dept Biotecnol, BR-12602810 Sao Paulo, Brazil
[2] Inst Politecn Nacl, ENCB, Mexico City 07738, DF, Mexico
[3] Inst Politecn Nacl, Unidad Profes Interdisciplinaria Biotecnol UPIBI, Mexico City 07738, DF, Mexico
来源：
BIORESOURCE TECHNOLOGY | 2016年 / 203卷
关键词：
Packed bed reactor; Reuse of alkaline liquor; Enzymatic saccharification; Bioethanol production; Sugarcane bagasse; ETHANOL-PRODUCTION; 2ND-GENERATION ETHANOL; HYDROLYSIS; DELIGNIFICATION; CELLULOSE; DIGESTIBILITY; FERMENTATION; SOLIDS; ALKALI; STRAW;
D O I：
10.1016/j.biortech.2015.12.026
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
A packed bed flow-through column reactor (PBFTCR) was used for pretreatment and subsequent enzymatic hydrolysis of sugarcane bagasse (SCB). Alkaline pretreatment was performed at 70 degrees C for 4 h with fresh 0.3 M NaOH solution or with liquor recycled from a previous pretreatment batch. Scheffersomyces stipitis NRRL-Y7124 was used for fermentation of sugars released after enzymatic hydrolysis (20 FPU g (1) of dry SCB). The highest results for lignin removal were 61% and 52%, respectively, observed when using fresh NaOH or the first reuse of the liquor. About 50% of cellulosic and 57% of hemicellulosic fractions of pretreated SCBs were enzymatically hydrolyzed and the maximum ethanol production was 23.4 g L (1) (ethanol yield of 0.4 g(p) g(s) (1)), with near complete consumption of both pentoses and hexoses present in the hydrolysate during the fermentation. PBFTCR as a new alternative for SCB-biorefineries is presented, mainly considering its simple configuration and efficiency for operating with a high solid: liquid ratio. (C) 2015 Elsevier Ltd. All rights reserved.
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收藏
页码：42 / 49
页数：8
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