Oxygenic photosynthesis without galactolipids

被引：50

作者：

Awai, Koichiro ^{[1
,2
,3
]}

Ohta, Hiroyuki ^{[4
,5
]}

Sato, Naoki ^{[6
,7
]}

机构：

[1] Shizuoka Univ, Grad Sch Sci, Shizuoka 4228529, Japan

[2] Shizuoka Univ, Res Inst Elect, Hamamatsu, Shizuoka 4328011, Japan

[3] Japan Sci & Technol Agcy, Precursory Res Embryon Sci & Technol, Kawaguchi, Saitama 3320012, Japan

[4] Tokyo Inst Technol, Ctr Biol Resources & Informat, Yokohama, Kanagawa 2268501, Japan

[5] Tokyo Inst Technol, Earth Life Sci Inst, Tokyo 1528551, Japan

[6] Japan Sci & Technol Agcy, Core Res Evolut Sci & Technol, Tokyo 1020076, Japan

[7] Univ Tokyo, Dept Life Sci, Grad Sch Arts & Sci, Tokyo 1538902, Japan

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2014年 / 111卷 / 37期

关键词：

chromatophore; cluster analysis; endosymbiosis; evolution of photosynthesis; NAD(P)H-dependent oxidoreductase; SP PCC 6803; MONOGLUCOSYLDIACYLGLYCEROL SYNTHASE; THYLAKOID MEMBRANES; PHOTOSYSTEM-II; SYNECHOCYSTIS; MONOGALACTOSYLDIACYLGLYCEROL; CYANOBACTERIA; BIOGENESIS; PROTEINS; ORIGIN;

D O I：

10.1073/pnas.1403708111

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The thylakoid membranes of oxygenic photosynthetic organisms are dominated by the galactolipids monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG). In cyanobacteria, MGDG is synthesized via monoglucosyldiacylglycerol (GlcDG). However, the putative epimerase involved in the conversion of GlcDG to MGDG has not been identified. Here we report the identification of the gene for the glucolipid epimerase (mgdE) by comparative genomic analysis. Knockout mutants of mgdE in Synechocystis sp. PCC 6803 lacked both MGDG and DGDG and accumulated GlcDG. The mutants did possess thylakoid membranes and showed normal maximal photosynthetic activity, albeit with reduced utilization of light energy. These results cast doubt on the long-standing belief that oxygenic photosynthesis is absolutely dependent on galactolipids.

引用

页码：13571 / 13575

页数：5

共 26 条

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