Updating the PGMY Primers and Probes for Improved Detection of HPV68a: Validation of Version 2 of the PGMY-CHUV Assay

被引：9
作者：
Estrade, C.
Sahli, R. [1 ]
机构：
[1] CHU Vaudois, Inst Microbiol, WHO HPV LabNet Reg Reference Lab Europe, Lausanne, Switzerland
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2014年 / 52卷 / 11期
关键词：
HYBRIDIZATION; PCR;
D O I：
10.1128/JCM.01950-14
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
HPV68a is not efficiently detected by PCR with the PGMY primers. Version 2 of the PGMY-CHUV assay (PGv2) was developed from version 1 (PGv1) to evaluate HPV68-discordant results with the Anyplex II HPV28 assay. We now report that PGv2 is significantly more sensitive than PGv1 for HPV68a and as sensitive and specific for the other HPV genotypes during a 1-year prospective validation (n = 714 samples).
引用
收藏
页码：4033 / 4035
页数：3
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