Submerged culture production of chitinase by Trichoderma harzianum in stirred tank bioreactors -: the influence of agitator speed

被引：43
作者：
Felse, PA [1 ]
Panda, T [1 ]
机构：
[1] Indian Inst Technol, Dept Chem Engn, Biotechnol Res Ctr, Madras 600036, Chennai, India
来源：
BIOCHEMICAL ENGINEERING JOURNAL | 2000年 / 4卷 / 02期
关键词：
agitation; bioreactors; chitinase; kinetics; Trichoderma harzianum; yield;
D O I：
10.1016/S1369-703X(99)00039-X
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The chitinase fermentation process utilizing chitin as the sole carbon source was investigated ina stirred tank bioreactor. Agitator speed of 224 rpm was found to be most suitable for cell growth as well as for chitinase production. Chitinase yield decreased rapidly at higher agitator speed, while decrease in cell yield at higher agitator speed was not rapid. Probably, mass transfer limitation was predominant in the fermentation process at lower agitator speed. Higher agitator speed appears to reduce chitinase production. (C) 2000 Elsevier Science S.A. All rights reserved.
引用
收藏
页码：115 / 120
页数：6
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