Multilaboratory Study of Epidemiological Cutoff Values for Detection of Resistance in Eight Candida Species to Fluconazole, Posaconazole, and Voriconazole

被引：92

作者：

Espinel-Ingroff, A. ^{[1
]}

Pfaller, M. A. ^{[2
,3
]}

Bustamante, B. ^{[4
]}

Canton, E. ^{[5
]}

Fothergill, A. ^{[6
]}

Fuller, J. ^{[7
]}

Gonzalez, G. M. ^{[8
]}

Lass-Floerl, C. ^{[9
]}

Lockhart, S. R. ^{[10
]}

Martin-Mazuelos, E. ^{[11
]}

Meis, J. F. ^{[12
,13
]}

Melhem, M. S. C. ^{[14
]}

Ostrosky-Zeichner, L. ^{[15
]}

Pelaez, T. ^{[16
]}

Szeszs, M. W. ^{[17
]}

St-Germain, G. ^{[18
]}

Bonefietti, L. X. ^{[19
]}

Guarro, J. ^{[20
]}

Turnidge, J. ^{[21
]}

机构：

[1] VCU Med Ctr, Richmond, VA USA

[2] JMI Labs, North Liberty, IA USA

[3] Univ Iowa, Coll Med, Iowa City, IA USA

[4] Univ Peruana Cayetano Heredia, Inst Med Trop Alexander Von Humboldt, Lima, Peru

[5] Hosp Univ La Fe, Ctr Invest, Unidad Microbiol Expt, Valencia, Spain

[6] Univ Texas Hlth Sci Ctr San Antonio, San Antonio, TX 78229 USA

[7] Univ Alberta, Edmonton, AB, Canada

[8] Univ Autonoma Nuevo Leon, Monterrey, Nuevo Leon, Mexico

[9] Med Univ Innsbruck, Div Hyg & Med Microbiol, A-6020 Innsbruck, Austria

[10] Ctr Dis Control & Prevent, Mycot Dis Branch, Atlanta, GA USA

[11] Hosp Univ Valme, Seville, Spain

[12] Canisius Wilhelmina Hosp, Nijmegen, Netherlands

[13] Radboud Univ Nijmegen, NL-6525 ED Nijmegen, Netherlands

[14] Adolfo Lutz Inst, Fungal Taxon Labs, Sao Paulo, Brazil

[15] Univ Texas Hlth Sci Ctr Houston, Houston, TX 77030 USA

[16] Univ Complutense, Fac Med, Hosp Gen Univ Gregorio Maranon, E-28040 Madrid, Spain

[17] Adolfo Lutz Inst, Dept Mycol, Sao Paulo, Brazil

[18] Inst Natl Sante Publ Quebec, Lab Sante Publ Quebec, Quebec City, PQ, Canada

[19] Adolfo Lutz Inst, Aracatuba City, Brazil

[20] IISPV, Fac Med, Reus, Spain

[21] Univ Adelaide, Adelaide, SA, Australia

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2014年 / 58卷 / 04期

关键词：

BROTH MICRODILUTION METHOD; AZOLE RESISTANCE; IN-VITRO; MIC DISTRIBUTIONS; ANTIFUNGAL AGENTS; AMPHOTERICIN-B; WILD-TYPE; SUSCEPTIBILITY; MECHANISMS; ITRACONAZOLE;

D O I：

10.1128/AAC.02615-13

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Although epidemiological cutoff values (ECVs) have been established for Candida spp. and the triazoles, they are based on MIC data from a single laboratory. We have established ECVs for eight Candida species and fluconazole, posaconazole, and voriconazole based on wild-type (WT) MIC distributions for isolates of C. albicans (n = 11,241 isolates), C. glabrata (7,538), C. parapsilosis (6,023), C. tropicalis (3,748), C. krusei (1,073), C. lusitaniae (574), C. guilliermondii (373), and C. dubliniensis (162). The 24-h CLSI broth microdilution MICs were collated from multiple laboratories (in Canada, Brazil, Europe, Mexico, Peru, and the United States). The ECVs for distributions originating from >= 6 laboratories, which included >= 95% of the modeled WT population, for fluconazole, posaconazole, and voriconazole were, respectively, 0.5, 0.06 and 0.03 mu g/ml for C. albicans, 0.5, 0.25, and 0.03 mu g/ml for C. dubliniensis, 8, 1, and 0.25 mu g/ml for C. glabrata, 8, 0.5, and 0.12 mu g/ml for C. guilliermondii, 32, 0.5, and 0.25 mu g/ml for C. krusei, 1, 0.06, and 0.06 mu g/ml for C. lusitaniae, 1, 0.25, and 0.03 mu g/ml for C. parapsilosis, and 1, 0.12, and 0.06 mu g/ml for C. tropicalis. The low number of MICs (<100) for other less prevalent species (C. famata, C. kefyr, C. orthopsilosis, C. rugosa) precluded ECV definition, but their MIC distributions are documented. Evaluation of our ECVs for some species/agent combinations using published individual MICs for 136 isolates (harboring mutations in or upregulation of ERG11, MDR1, CDR1, or CDR2) and 64 WT isolates indicated that our ECVs may be useful in distinguishing WT from non-WT isolates.

引用

页码：2006 / 2012

页数：7

共 41 条

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