Sample holder for axial rotation of specimens in 3D microscopy

被引：27

作者：

Bruns, T. ^{[1
]}

Schickinger, S. ^{[1
]}

Schneckenburger, H. ^{[1
]}

机构：

[1] Aalen Univ, Inst Appl Res, D-73430 Aalen, Germany

来源：

JOURNAL OF MICROSCOPY | 2015年 / 260卷 / 01期

关键词：

3D imaging; axial tomography; confocal laser scanning microscopy; light sheet fluorescence microscopy; microcapillary; SPIM; PLANE ILLUMINATION MICROSCOPY; LIGHT-SHEET MICROSCOPE; FLUORESCENCE MICROSCOPY; OPTICAL MICROSCOPY;

D O I：

10.1111/jmi.12263

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

In common light microscopy, observation of samples is only possible from one perspective. However, especially for larger three-dimensional specimens observation from different views is desirable. Therefore, we are presenting a sample holder permitting rotation of the specimen around an axis perpendicular to the light path of the microscope. Thus, images can be put into a defined multidimensional context, enabling reliable three-dimensional reconstructions. The device can be easily adapted to a great variety of common light microscopes and is suitable for various applications in science, education and industry, where the observation of three-dimensional specimens is essential. Fluorescence z-projection images of copepods and ixodidae ticks at different rotation angles obtained by confocal laser scanning microscopy and light sheet fluorescence microscopy are reported as representative results.

引用

页码：30 / 36

页数：7

共 25 条

[1] The natural history of ticks [J].