Homologous recombination in human embryonic stem cells using CRISPR/Cas9 nickase and a long DNA donor template

被引:58
作者
Rong, Zhili [2 ]
Zhu, Shengyun [1 ]
Xu, Yang [2 ]
Fu, Xuemei [1 ,3 ]
机构
[1] Shenzhen Childrens Hosp, Shenzhen 518026, Peoples R China
[2] Univ Calif San Diego, Div Biol Sci, La Jolla, CA 92093 USA
[3] Chongqing Med Univ, Chongqing 400016, Peoples R China
关键词
HUMAN ESCS; GENOME; CAS9; SPECIFICITY; NUCLEASES; GENERATION; SYSTEM; GENES; MICE;
D O I
10.1007/s13238-014-0032-5
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
引用
收藏
页码:258 / 260
页数:3
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