Frequency-doubled femtosecond Er-doped fiber laser for two-photon excited fluorescence imaging

被引：20

作者：

Stachowiak, Dorota ^{[1
]}

Boguslawski, Jakub ^{[2
]}

Gluszek, Aleksander ^{[1
]}

Laszczych, Zbigniew ^{[1
]}

Wojtkowski, Maciej ^{[2
]}

Sobon, Grzegorz ^{[1
]}

机构：

[1] Wroclaw Univ Sci & Technol, Laser & Fiber Elect Grp, Fac Elect, Wybrzeze Wyspianskiego 27, PL-50370 Wroclaw, Poland

[2] Polish Acad Sci, Int Ctr Translat Eye Res, Inst Phys Chem, Kasprzaka 44-52, PL-01224 Warsaw, Poland

来源：

BIOMEDICAL OPTICS EXPRESS | 2020年 / 11卷 / 08期

基金：

欧盟地平线“2020”;

关键词：

SELF-PHASE-MODULATION; SOLITON COMPRESSION; PULSE GENERATION; REPETITION-RATE; MICROSCOPY; DIODE;

D O I：

10.1364/BOE.396878

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A femtosecond frequency-doubled erbium-doped fiber laser with an adjustable pulse repetition rate is developed and applied in two-photon excited fluorescence microscopy. The all-fiber laser system provides the fundamental pulse at 1560 nm wavelength with 22 fs duration for the second harmonic generation, resulting in 1.35 nJ, 60 fs pulses at 780 nm. The repetition rate is adjusted by a pulse picker unit built-in within the amplifier chain, directly providing transform-limited pulses for any chosen repetition rate between 1 and 12 MHz. We employed the laser source to drive a scanning two-photon excited fluorescence microscope for ex vivo rat skin and other samples' imaging at various pulse repetition rates. Due to compactness, ease of operation, and suitable pulse characteristics, the laser source can be considered as an attractive alternative for Ti:Sapphire laser in biomedical imaging. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

引用

页码：4431 / 4442

页数：12

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