Development and robustness testing of a nonaqueous capillary electrophoresis method for the analysis of nonsteroidal anti-inflammatory drugs

被引：48

作者：

Cherkaoui, S ^{[1
]}

Veuthey, JL ^{[1
]}

机构：

[1] Univ Geneva, Lab Pharmaceut Analyt Chem, Pavill Isotopes, CH-1211 Geneva 4, Switzerland

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 874卷 / 01期

关键词：

nonaqueous capillary electrophoresis; background electrolyte composition; pharmaceutical analysis; nonsteroidal anti-inflammatory drugs;

D O I：

10.1016/S0021-9673(00)00052-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nine non steroidal anti-inflammatory drugs were simultaneously separated by nonaqueous capillary electrophoresis with a methanol-acetonitrile (40:60, v/v) mixture containing 20 mM ammonium acetate. The effect of solvent composition, electrolyte nature and concentration on the electrophoretic behavior of the selected drugs was systematically studied. Investigated electrolytes were ammonium, lithium and sodium acetate. Modification of the solvent and/or the electrolyte composition was found to alter the migration order of the pharmaceutical drugs. Finally, to assess method robustness, three sensitive electrophoretic parameters as well as their interactions were evaluated using a full factorial design at two levels. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：121 / 129

页数：9

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