Cytotoxic, Anti-Proliferative and Apoptosis Activity of L-Amino Acid Oxidase from Malaysian Cryptelytrops purpureomaculatus (CP-LAAO) Venom on Human Colon Cancer Cells

被引：18

作者：

Abidin, Syafiq Asnawi Zainal ^{[1
]}

Rajadurai, Pathmanathan ^{[1
]}

Chowdhury, Md. Ezharul Hoque ^{[1
]}

Othman, Iekhsan ^{[1
]}

Naidu, Rakesh ^{[1
]}

机构：

[1] Monash Univ Malaysia, Jeffrey Cheah Sch Med & Hlth Sci, Jalan Lagoon Selatan, Bandar Sunway 47500, Selangor Darul, Malaysia

来源：

MOLECULES | 2018年 / 23卷 / 06期

关键词：

L-amino acid oxidase; Cryptelytrops purpureomaculatus; cytotoxicity; apoptosis; human colon cancer cells; ACUTUS SNAKE-VENOM; STRUCTURAL-CHARACTERIZATION; CALLOSELASMA-RHODOSTOMA; PURIFICATION; SUBSTRATE;

D O I：

10.3390/molecules23061388

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study is to investigate the potential anti-cancer activity of L-amino acid oxidase (CP-LAAO) purified from the venom of Cryptelytrops purpureomaculatus on SW480 and SW620 human colon cancer cells. Mass spectrometry guided purification was able to identify and purify CP-LAAO. Amino acid variations identified from the partial protein sequence of CP-LAAO may suggest novel variants of these proteins. The activity of the purified CP-LAAO was confirmed with o-phenyldiamine (OPD)-based spectrophotometric assay. CP-LAAO demonstrated time-and dose-dependent cytotoxic activity and the EC50 value was determined at 13 mu g/mL for both SW480 and SW620 cells. Significant increase of caspase-3 activity, reduction of Bcl-2 levels, as well as morphological changes consistent with apoptosis were demonstrated by CP-LAAO. Overall, these data provide evidence on the potential anti-cancer activity of CP-LAAO from the venom of Malaysian C. purpureomaculatus for therapeutic intervention of human colon cancer.

引用

页数：16

共 36 条

[1] Proteomic Characterization and Comparison of Malaysian Tropidolaemus wagleri and Cryptelytrops purpureomaculatus Venom Using Shotgun-Proteomics [J].

Abidin, Syafiq Asnawi Zainal ;

Rajadurai, Pathmanathan ;

Chowdhury, Md Ezharul Hoque ;

Rusmili, Muhamad Rusdi Ahmad ;

Othman, Iekhsan ;

Naidu, Rakesh .

TOXINS, 2016, 8 (10)

[2] Models for prevention and treatment of cancer: Problems vs promises [J].

Aggarwal, Bharat B. ;

Danda, Divya ;

Gupta, Shan ;

Gehlot, Prashasnika .

BIOCHEMICAL PHARMACOLOGY, 2009, 78 (09) :1083-1094

[3] Characterization and cytotoxicity of L-amino acid oxidase from the venom of king cobra (Ophiophagus hannah) [J].

Ahn, MY ;

Lee, BM ;

Kim, YS .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 1997, 29 (06) :911-919

[4] Isolation, structural, and functional characterization of an apoptosis-inducing L-amino acid oxidase from leaf-nosed viper (Eristocophis macmahoni) snake venom [J].

Ali, SA ;

Stoeva, S ;

Abbasi, A ;

Alam, JM ;

Kayed, R ;

Faigle, M ;

Neumeister, B ;

Voelter, W .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2000, 384 (02) :216-226

[5] Evidence of caspase-mediated apoptosis induced by L-amino acid oxidase isolated from Bothrops atrox snake venom [J].

Alves, Raquel Melo ;

Antonucci, Gilmara Ausech ;

Paiva, Helder Henrique ;

Cintra, Adelia Cristina Oliveira ;

Franco, Joao Jose ;

Mendonca-Franqueiro, Elaine Paula ;

Dorta, Daniel Junqueira ;

Giglio, Jose Roberto ;

Rosa, Jose Cesar ;

Fuly, Andre Lopes ;

Dias-Baruffi, Marcelo ;

Soares, Andreirnar Martins ;

Sampaio, Suely Vilela .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 2008, 151 (04) :542-550

[6] Mechanisms of cell death induction by L-amino acid oxidase, a major component of ophidian venom [J].

Ande, Sudharsana Rao ;

Kommoju, Phaneeswara Rao ;

Draxl, Sigrid ;

Murkovic, Michael ;

Macheroux, Peter ;

Ghisla, Sandro ;

Ferrando-May, Elisa .

APOPTOSIS, 2006, 11 (08) :1439-1451

[7]

Bardhan Kankana, 2013, Cancers (Basel), V5, P676, DOI 10.3390/cancers5020676

[8] Cancer and Radiation Therapy: Current Advances and Future Directions [J].

Baskar, Rajamanickam ;

Lee, Kuo Ann ;

Yeo, Richard ;

Yeoh, Kheng-Wei .

INTERNATIONAL JOURNAL OF MEDICAL SCIENCES, 2012, 9 (03) :193-199

[9] UniProt: the universal protein knowledgebase [J].

Bateman, Alex ;

Martin, Maria Jesus ;

O'Donovan, Claire ;

Magrane, Michele ;

Alpi, Emanuele ;

Antunes, Ricardo ;

Bely, Benoit ;

Bingley, Mark ;

Bonilla, Carlos ;

Britto, Ramona ;

Bursteinas, Borisas ;

Bye-A-Jee, Hema ;

Cowley, Andrew ;

Da Silva, Alan ;

De Giorgi, Maurizio ;

Dogan, Tunca ;

Fazzini, Francesco ;

Castro, Leyla Garcia ;

Figueira, Luis ;

Garmiri, Penelope ;

Georghiou, George ;

Gonzalez, Daniel ;

Hatton-Ellis, Emma ;

Li, Weizhong ;

Liu, Wudong ;

Lopez, Rodrigo ;

Luo, Jie ;

Lussi, Yvonne ;

MacDougall, Alistair ;

Nightingale, Andrew ;

Palka, Barbara ;

Pichler, Klemens ;

Poggioli, Diego ;

Pundir, Sangya ;

Pureza, Luis ;

Qi, Guoying ;

Rosanoff, Steven ;

Saidi, Rabie ;

Sawford, Tony ;

Shypitsyna, Aleksandra ;

Speretta, Elena ;

Turner, Edward ;

Tyagi, Nidhi ;

Volynkin, Vladimir ;

Wardell, Tony ;

Warner, Kate ;

Watkins, Xavier ;

Zaru, Rossana ;

Zellner, Hermann ;

Xenarios, Ioannis .

NUCLEIC ACIDS RESEARCH, 2017, 45 (D1) :D158-D169

[10] Isolation and biochemical, functional and structural characterization of a novel L-amino acid oxidase from Lachesis muta snake venom [J].

Bregge-Silva, Cristiane ;

Nonato, Maria Cristina ;

de Albuquerque, Sergio ;

Ho, Paulo Lee ;

Junqueira de Azevedo, Inacio L. M. ;

Vasconcelos Diniz, Marcelo Ribeiro ;

Lomonte, Bruno ;

Rucavado, Alexandra ;

Diaz, Cecilia ;

Maria Gutierrez, Jose ;

Arantes, Eliane Candiani .

TOXICON, 2012, 60 (07) :1263-1276

← 1 2 3 4 →