HYALURONIDASE PRETREATMENT IMPROVES THE CRYOPRESERVATION OF HUMAN OVARIAN TISSUE

被引：0

作者：

Xiao, Zhun ^{[1
]}

Huang, Zhong-Ying ^{[1
]}

Zhang, Yao-Yao ^{[1
]}

Fan, Wei ^{[1
]}

Chen, Qiong ^{[2
]}

机构：

[1] Sichuan Univ, Minist Educ, West China Univ Hosp 2,Reprod Med Ctr, Key Lab Birth Defects & Related Dis Women & Child, Chengdu 610041, Sichuan, Peoples R China

[2] Sichuan Univ, West China Hosp 2, Neonatal Intens Care Unit, Chengdu 610041, Sichuan, Peoples R China

来源：

CRYOLETTERS | 2019年 / 40卷 / 03期

关键词：

human ovarian tissue; slow freezing; primordial follicle; hyaluronidase; steroid hormone; FERTILITY PRESERVATION; TRANSPLANTATION; VITRIFICATION; CHILDHOOD; FOLLICLES; CHILDREN; PATIENT; GIRLS;

D O I：

暂无

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

BACKGROUND: Slow freezing has been widely applied to preserve human ovarian tissue. OBJECTIVE: The aim of this study is to determine whether human ovarian cortex pretreated with hyaluronidase can improve the protective effect of cryopreservation. MATERIALS AND METHODS: Human ovarian biopsies from seven patients were cryopreserved using slow freezing. The samples in the experimental group were incubated in culture medium with hyaluronidase for a short time before cryopreservation. The histology of the thawed ovarian tissue was investigated; the levels of steroid hormone in the culture media were then used to evaluate the development and function of thawed ovarian tissue. RESULTS: The result showed that the percentage of morphologically abnormal primordial follicles was higher in the freezing control than in the experimental group (P<0.05). 17 beta-estradiol (E-2) and progesterone (P-4) concentrations were significantly higher in the experimental group than in the freezing control group (P<0.05). CONCLUSION: Our study showed that human ovarian cortex pretreatment with hyaluronidase can improve the protective effect of cryopreservation by improving the penetration of cryoprotectant.

引用

页码：139 / 144

页数：6

共 24 条

[1]

Alberts B., 2002, Molecular Biology of the Cell, V4th

[2] Two successful pregnancies following autotransplantation of frozen/thawed ovarian tissue [J].

Andersen, Claus Yding ;

Rosendahl, Mikkel ;

Byskov, Anne Grete ;

Loft, Anne ;

Ottosen, Christian ;

Dueholm, Margit ;

Schmidt, Kirsten L. T. ;

Andersen, Anders Nyboe ;

Ernst, Erik .

HUMAN REPRODUCTION, 2008, 23 (10) :2266-2272

[3] Fertility preservation in girls and young women [J].

Anderson, Richard A. ;

Wallace, W. Hamish B. .

CLINICAL ENDOCRINOLOGY, 2011, 75 (04) :409-419

[4] Optimal condition of vitrification method for cryopreservation of human ovarian cortical tissues [J].

Chang, Hye Jin ;

Moon, Jeong Hee ;

Lee, Jung Ryeol ;

Jee, Byung Chul ;

Suh, Chang Suk ;

Kim, Seok Hyun .

JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH, 2011, 37 (08) :1092-1101

[5]

Christiani A, 2009, HUMAN REPROD, V24, P92

[6] Fertility preservation: Successful transplantation of cryopreserved ovarian tissue in a young patient previously treated for Hodgkin's disease [J].

Demeestere, Isabelle ;

Simon, Philippe ;

Emiliani, Serena ;

Delbaere, Anne ;

Englert, Yvon .

ONCOLOGIST, 2007, 12 (12) :1437-1442

[7] Livebirth after orthotopic transplantation of cryopreserved ovarian tissue [J].

Donnez, J ;

Dolmans, MM ;

Demylle, D ;

Jadoul, P ;

Pirard, C ;

Squifflet, J ;

Martinez-Madrid, B ;

Van Langendonckt, A .

LANCET, 2004, 364 (9443) :1405-1410

[8]

Donnez J., 2011, FERTIL STERIL, V95, P1787, DOI DOI 10.1016/J.FERTNSTERT.2010.11.041

[9] Fertility preservation in women [J].

Donnez, Jacques ;

Dolmans, Marie-Madeleine .

NATURE REVIEWS ENDOCRINOLOGY, 2013, 9 (12) :735-749

[10] The first woman to give birth to two children following transplantation of frozen/thawed ovarian tissue [J].

Ernst, Erik ;

Bergholdt, Stinne ;

Jorgensen, Jan Stener ;

Andersen, Claus Yding .

HUMAN REPRODUCTION, 2010, 25 (05) :1280-1281

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