Genome sequencing and annotation of multi-virulent Aeromonas veronii XhG1.2 isolated from diseased Xiphophorus hellerii

被引：10
作者：
Das, Soumya [1 ]
Sreejith, S. [1 ]
Babu, Jilna [2 ]
Francis, Celen [2 ]
Midhun, J. S. [1 ]
Aswani, R. [1 ]
Sebastain, K. S. [3 ]
Radhakrishnan, E. K. [1 ]
Mathew, Jyothis [1 ]
机构：
[1] Mahatma Gandhi Univ, Sch Biosci, PD Hills PO, Kottayam 686560, Kerala, India
[2] Mahatma Gandhi Univ, Ctr Bioinformat, PD Hills PO, Kottayam 686560, Kerala, India
[3] Govt Coll, Dept Zool, Kottayam 686013, Kerala, India
来源：
GENOMICS | 2021年 / 113卷 / 01期
关键词：
Whole genome sequencing; Aeromonas veronii XhG1.2; Aerolysin; Hemolysin III; Antibiotic resistance;
D O I：
10.1016/j.ygeno.2020.10.034
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The present study was intended to elucidate the genomic basis of antibiotic resistance and hyper-virulence of the fish pathogen Aeromonas veronii XhG1.2 characterized in our previous work. The identity of XhG1.2 was confirmed through 16S rDNA sequence analysis and whole genome sequence analysis. The top-hit species distribution analysis of XhG1.2 sequence data revealed major hits against the Aeromonas veronii. The identification of virulence genes using the VFDB showed the genome of XhG1.2 to have the genes coding for the virulence factors viz. aerolysin, RtxA, T2SS, T3SS and T6SS. The presence of antibiotic resistance predicted through the CARD database analysis showed it to have the CephA3, OXA-12, adeF and pulvomycin resistance genes. By the phylogenetic and comparative genomic analysis, A. veronii species were found to have genes for toxin production. This also confirmed the pathogenicity and drug resistance of A. veronii XhG1.2 and also its potential to cause disease in diverse ornamental fishes.
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页码：991 / 998
页数：8
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