Bone marrow cell trafficking following intravenous administration

被引:66
作者
Cui, JS
Wahl, RL
Shen, TL
Fisher, SJ
Recker, E
Ginsburg, D
Long, MW
机构
[1] Univ Michigan, Howard Hughes Med Inst, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Dept Human Genet, Ann Arbor, MI 48109 USA
[3] Univ Michigan, Dept Internal Med, Ann Arbor, MI 48109 USA
[4] Univ Michigan, Dept Pediat, Ann Arbor, MI 48109 USA
[5] Univ Michigan, Dept Nucl Med, Ann Arbor, MI 48109 USA
[6] Univ Michigan, Ctr Comprehens Canc, Ann Arbor, MI 48109 USA
[7] George Washington Univ, Dept Pathol, Ann Arbor, MI USA
关键词
homing; bone marrow; haemopoiesis; cell migration; transplantation;
D O I
10.1046/j.1365-2141.1999.01779.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
To address trafficking of transplanted marrow cells immediately after intravenous infusion, we examined the early fate of infused non-adherent, low-density donor bone marrow cells in a syngeneic mouse model. The presence of infused donor cells, marked with indium-111 oxine (In-111), with the fluorescent dye PKH26, or by a detectable transgene marker, was evaluated at 3-48 h in a variety of tissues, including peripheral blood, All three cell-marking methods indicated a rapid (< 4 h) influx of cells into the bone marrow, liver, spleen, muscle and other tissues. Moreover, these tissues remained positive for the 48 h observation period. Interestingly, analysis of PKH26-positive cells in non-myeloablated animals demonstrated that approximately 17% of infused donor marrow cells localized to the marrow space within 15 h, whereas a smaller proportion of donor cells (similar to 1-2%) localized to the marrow in recipients preconditioned by irradiation, In an effort to enrich for cells that specifically home to the bone marrow, PKH26-labelled donor marrow cells were recovered from the first host and infused into a secondary recipient. Although this was a phenotypically undefined population of cells, no increase was observed in the relative fraction of PKH26-labelled cells returning or 'homing' to the marrow of the second recipient. Taken together, these data suggest both that marrow engraftment may be mediated by non-specific 'seeding' rather than a specific homing signal, and that efficient targeting of transplanted cells to the marrow is a complex multifaceted process.
引用
收藏
页码:895 / 902
页数:8
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