Fabrication of an amperometric sarcosine biosensor based on sarcosine oxidase/chitosan/CuNPs/c-MWCNT/Au electrode for detection of prostate cancer

被引:57
作者
Narwal, Vinay [1 ]
Kumar, Parveen [2 ]
Joon, Pooja [3 ]
Pundir, C. S. [1 ]
机构
[1] Maharshi Dayanand Univ, Dept Biochem, Rohtak, Haryana, India
[2] Maharshi Dayanand Univ, Dept Zool, Rohtak, Haryana, India
[3] Deen Bandhu Chhotu Ram Univ Sci & Technol, Sonepat, Haryana, India
关键词
Sarcosine; Sarcosine biosensor; Sarcosine oxidase; Nanomaterials; Serum; Prostate cancer; NANOPARTICLES; OXIDASE; GRAPHENE; SENSOR; ACID;
D O I
10.1016/j.enzmictec.2018.02.010
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
An amperometric sarcosine biosensor was fabricated based on covalent immobilization of sarcosine oxidase (SarOx) onto the nanocomposite of carboxylated multi -walled carbon nanotubes (cMWCNT)/chitosan (CHIT) and copper nanoparticles (CuNPs), electrodeposited on gold (Au) electrode. The SarOx/CHIT/CuNPs/cMWCNT/Au electrode was characterized by scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The enzyme electrode exhibited optimum current within 2 s at a potential of 0.2 V against Ag/AgCI, pH 7.0 and 35 degrees C. A linear relationship was obtained between sarcosine concentration in the range, 0.1-100 mu M and current (mA) under optimum conditions. The biosensor exhibited a high sensitivity of 277.5 mu A/mu M/cm(2), a low detection limit of 0.1 pM and excellent storage stability (180 days). The analytical recoveries of added sarcosine in sera at 0.5 mu M and at 1.0 mu M concentration were 95.5% and 97.30 respectively. The precision i.e. within and between-batch coefficients of variation (CVs) were 1.08% and 1.70% respectively. There was a good correlation (R-2 = 0.99) between the level of sarcosine in sera as measured by the standard immuno kit method and the present biosensor. The biosensor measured sarcosine level in sera of prostate cancer patients, which was significantly higher than those of apparently healthy persons (p value < 0.01).
引用
收藏
页码:44 / 51
页数:8
相关论文
共 30 条
[1]   SERUM BETAINE, N,N-DIMETHYLGLYCINE AND N-METHYLGLYCINE LEVELS IN PATIENTS WITH COBALAMIN AND FOLATE-DEFICIENCY AND RELATED INBORN-ERRORS OF METABOLISM [J].
ALLEN, RH ;
STABLER, SP ;
LINDENBAUM, J .
METABOLISM-CLINICAL AND EXPERIMENTAL, 1993, 42 (11) :1448-1460
[2]  
[Anonymous], BIOCH ANAL BIOCH
[3]   Synthesis of multiwall carbon nanotubes by electric arc discharge in liquid environments [J].
Antisari, MV ;
Marazzi, R ;
Krsmanovic, R .
CARBON, 2003, 41 (12) :2393-2401
[4]   An amperometric lactate biosensor based on lactate dehydrogenase immobilized onto graphene oxide nanoparticles-modified pencil graphite electrode [J].
Batra, Bhawna ;
Narwal, Vinay ;
Pundir, Chandra S. .
ENGINEERING IN LIFE SCIENCES, 2016, 16 (08) :786-794
[5]   A novel enzymatic technique for determination of sarcosine in urine samples [J].
Burton, Casey ;
Gamagedara, Sanjeewa ;
Ma, Yinfa .
ANALYTICAL METHODS, 2012, 4 (01) :141-146
[6]  
Cernei N, 2012, INT J ELECTROCHEM SC, V7, P4286
[7]  
Couzin J, 2009, SCIENCE, V323, P865, DOI 10.1126/science.323.5916.865a
[8]  
HASHIMOTO K, 2009, OPEN CLIN CHEM J, V2, P22, DOI DOI 10.2174/1874241600902010022
[9]   Paramagnetic Nanoparticles as a Platform for FRET-Based Sarcosine Picomolar Detection [J].
Heger, Zbynek ;
Cernei, Natalia ;
Krizkova, Sona ;
Masarik, Michal ;
Kopel, Pavel ;
Hodek, Petr ;
Zitka, Ondrej ;
Adam, Vojtech ;
Kizek, Rene .
SCIENTIFIC REPORTS, 2015, 5
[10]   Nanomaterials for Biosensing Applications [J].
Hou, Sichao ;
Zhang, Aiying ;
Su, Ming .
NANOMATERIALS, 2016, 6 (04)