Silencing of CDC42 inhibits neuroblastoma cell proliferation and transformation

被引：26
作者：
Lee, Sora [1 ]
Craig, Brian T. [1 ]
Romain, Carmelle V. [1 ]
Qiao, Jingbo [1 ]
Chung, Dai H. [1 ,2 ]
机构：
[1] Vanderbilt Univ, Dept Pediat Surg, Med Ctr, Nashville, TN 37232 USA
[2] Vanderbilt Univ, Dept Canc Biol, Med Ctr, Nashville, TN 37232 USA
来源：
CANCER LETTERS | 2014年 / 355卷 / 02期
基金：
美国国家卫生研究院;
关键词：
CDC42; AKT2; Twist; N-myc; Transformation; Neuroblastoma; CANCER-CELLS; N-MYC; COLORECTAL-CANCER; TUMOR-METASTASIS; UP-REGULATION; RHO-GTPASES; TWIST; INVASION; DIFFERENTIATION; MORPHOGENESIS;
D O I：
10.1016/j.canlet.2014.08.033
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Cell division cycle 42 (CDC42), a small GTPase of the Rho-subfamily, regulates diverse cellular functions including proliferation, cytoskeletal rearrangement and even promotes malignant transformation. Here, we found that increased expression of CDC42 correlated with undifferentiated neuroblastoma as compared to a more benign phenotype. CDC42 inhibition decreased cell growth and soft agar colony formation, and increased cell death in BE(2)-C and BE(2)-M17 cell lines, but not in SK-N-AS. In addition, silencing of CDC42 decreased expression of N-myc in BE(2)-C and BE(2)-M17 cells. Our findings suggest that CDC42 may play a role in the regulation of aggressive neuroblastoma behavior. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：210 / 216
页数：7
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