Complete assignment of 1H and 13C NMR spectra of Gigartina skottsbergii λ-carrageenan using carrabiose oligosaccharides prepared by enzymatic hydrolysis

被引：48
作者：
Guibet, Marion
Kervarec, Nelly
Genicot, Sabine
Chevolot, Yann
Helbert, William [1 ]
机构：
[1] UPMC, CNRS, UMR 7139, Stn Biol, F-29680 Roscoff, France
[2] Univ Bretagne Occidentale, Serv Commun Resonance Magnet Nucl, F-29200 Brest, France
来源：
CARBOHYDRATE RESEARCH | 2006年 / 341卷 / 11期
关键词：
lambda-Carrageenan; carrageenase; Gigartina skottsbergii; hybrid structure;
D O I：
10.1016/j.carres.2006.04.018
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
lambda-Carrageenan extracted from Gigartina skottsbergii tetrasporophyte was completely digested by a purified Pseudoalteromonas carrageenovora X-carrageenase. The main digestion products were fractionated and analysed by H-1 and C-13 NMR spectroscopy. All the oligosaccharides observed belong to the neo-carrabiose oligosaccharide series indicating that the X-carrageenase cleaves the beta-(1 -> 4) glycosidic bonds. H-1 and C-13 NMR spectra recorded on oligomers from DP 2 to DP 8 were fully interpreted allowing unambiguous assignment of the lambda-carrageenan spectra. Besides the typical oligo-lambda-carrageenans, we have also characterised a heptasulfated tetrasaccharide which demonstrates the random over-sulfation along the chain of G. skottsbergii X-carrageenan. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1859 / 1869
页数：11
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