Differential Expression of Leukocyte Functions Associated Antigen-1 (LFA-1) on Peripheral Blood Mononuclear Cells in Patients with Crohn's Disease

被引：0

作者：

Yada, Shinichiro ^{[1
]}

Matsumoto, Takayuki ^{[1
]}

Esaki, Motohiro ^{[1
]}

Jo, Yukihiko ^{[1
]}

Koga, Hideki ^{[1
]}

Nakamura, Shotaro ^{[1
]}

Iida, Mitsuo ^{[1
]}

机构：

[1] Kyushu Univ, Grad Sch Med Sci, Dept Med & Clin Sci, Higashi Ku, Fukuoka 8130031, Japan

来源：

INFLAMMATORY BOWEL DISEASES | 2009年 / 15卷 / 09期

基金：

日本学术振兴会;

关键词：

Crohn's disease; LFA-1; infliximab; total parenteral nutrition; Crohn's Disease Activity Index; NECROSIS-FACTOR-ALPHA; T-CELLS; MONOCLONAL-ANTIBODY; ADHESION MOLECULES; ULCERATIVE-COLITIS; LAMINA PROPRIA; TNF-ALPHA; ACTIVATION; INFLIXIMAB; ICAM-1;

D O I：

10.1002/ibd.20935

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background: The leukocyte Function associated antigen-1 (LFA-1) intracellular adhesion molecule-1 pathway is presumed to play a pivotal role in the perpetuation of inflammatory bowel disease. We aimed to elucidate the effect of 2 different therapies on LFA-1 expression in patients with Crohn's disease (CD) and correlate LFA-1 expression with disease activity. Methods: fit all, 30 patients with active CD were recruited for the present investigation. Eleven patients were treated with infliximab and 19 patients with total parenteral nutrition. The clinical activity and the expression of LFA-1 in peripheral blood mononuclear cells were assessed prior to and 4 weeks after treatment. Clinical activity was determined by measuring the Crohn's Disease Activity Index and LFA-1 expression was measured by mean fluorescence intensity (MFI) under fluorescence-activated cell sorter analysis. Results: In each treatment group the clinical disease activity index decreased significantly 4 weeks after treatment. In patients treated with infliximab, LFA-1 expression decreased significantly (mean MFI decreased from 1983 to 1487, P < 0.05). However, LFA-1 expression remained unchanged in the total parenteral nutrition group (mean MFI elevated from 1684 to 1902, P > 0.05), Conclusions: The mechanism of therapeutic action on CD is different between infliximab and total parenteral nutrition.

引用

页码：1379 / 1384

页数：6

共 35 条

[11] TNF-α blockade down-regulates the CD40/CD40L pathway in the mucosal microcirculation:: A novel anti-inflammatory mechanism of infliximab in Crohn's disease [J].

Danese, S ;

Sans, M ;

Scaldaferri, F ;

Sgambato, A ;

Rutella, S ;

Cittadini, A ;

Piqué, JM ;

Panes, J ;

Katz, JA ;

Gasbarrini, A ;

Fiocchi, C .

JOURNAL OF IMMUNOLOGY, 2006, 176 (04) :2617-2624

[12]

Deeths MJ, 1999, EUR J IMMUNOL, V29, P45, DOI 10.1002/(SICI)1521-4141(199901)29:01<45::AID-IMMU45>3.0.CO

[13]

2-I

[14] Increased resistance of LFA-1-deficient mice to lipopolysaccharide-induced shock/liver injury in the presence of TNF-α and IL-12 is mediated by IL-10:: A novel role for LFA-1 in the regulation of the proinflammatory and anti-inflammatory cytokine balance [J].

Emoto, M ;

Emoto, Y ;

Brinkmann, V ;

Miyamoto, M ;

Yoshizawa, I ;

Stäber, M ;

van Rooijen, N ;

Hamann, A ;

Kaufmann, SHE .

JOURNAL OF IMMUNOLOGY, 2003, 171 (02) :584-593

[15] Dynamic shifts in LFA-1 affinity regulate neutrophil rolling, arrest, and transmigration on inflamed endothelium [J].

Green, CE ;

Schaff, UY ;

Sarantos, MR ;

Lum, AFH ;

Staunton, DE ;

Simon, SI .

BLOOD, 2006, 107 (05) :2101-2111

[16] Maintenance infliximab for Crohn's disease: the ACCENT I randomised trial [J].

Hanauer, SB ;

Feagan, BG ;

Lichtenstein, GR ;

Mayer, LF ;

Schreiber, S ;

Colombel, JF ;

Rachmilewitz, D ;

Wolf, DC ;

Olson, A ;

Bao, WH ;

Rutgeerts, P .

LANCET, 2002, 359 (9317) :1541-1549

[17] Effects of infliximab on cytokines, myeloperoxidase, and soluble adhesion molecules in patients with juvenile idiopathic arthritis [J].

Levalampi, T. ;

Honkanen, V. ;

Lahdenne, P. ;

Nieminen, R. ;

Hakala, M. ;

Moilanen, E. .

SCANDINAVIAN JOURNAL OF RHEUMATOLOGY, 2007, 36 (03) :189-193

[18] Vitamin D derivatives induce apoptosis and downregulate ICAM-1 levels in peripheral blood mononuclear cells of inflammatory bowel disease patients [J].

Martinesi, Maria ;

Treves, Cristina ;

d'Albasio, Giuseppe ;

Bagnoli, Siro ;

Bonanomi, Andrea G. ;

Stio, Maria .

INFLAMMATORY BOWEL DISEASES, 2008, 14 (05) :597-604

[19] Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling [J].

Matsumoto, M ;

Kikkawa, S ;

Kohase, M ;

Miyake, K ;

Seya, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 293 (05) :1364-1369

[20] Three-dimensional segregation of supramolecular activation clusters in T cells [J].

Monks, CRF ;

Freiberg, BA ;

Kupfer, H ;

Sciaky, N ;

Kupfer, A .

NATURE, 1998, 395 (6697) :82-86

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