Use of the pAL5000 replicon in PAH-degrading mycobacteria: application for strain labelling and promoter probing

被引：15

作者：

Wattiau, P

Springael, D

Agathos, SN

Wuertz, S

机构：

[1] Univ Catholique Louvain, Bioengn Unit, B-1348 Louvain, Belgium

[2] Vlaamse Instelling Technol Onderzoek, B-2400 Mol, Belgium

[3] Tech Univ Munich, Inst Water Qual Control & Waste Management, D-85748 Garching, Germany

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2002年 / 59卷 / 06期

关键词：

D O I：

10.1007/s00253-002-1081-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Three environmental Mycobacterium strains (LB501T, LB307T and VM552) able to degrade anthracene, phenanthrene or pyrene, respectively, were successfully electroporated with pAL5000-based plasmids containing the green fluorescent protein (gfp) gene of Aequoria victoria under the control of the hsp60 promoter of Mycobacterium bovis following a slightly modified standard procedure. Transformants showed irregular gfp expression profiles. Four plasmid derivatives were constructed that contained gene promoters isolated from, and adapted to, gene expression in polycyclic aromatic hydrocarbon (PAH)-degrading mycobacteria. One derivative directed strong and homogeneous expression of GFP, allowing dual analysis of both GFP- and PAH-derived fluorescence as assessed by confocal laser scanning microscopy. The results reported here demonstrate the suitability of the pAL5000 replicon for the development of recombinant DNA-based studies in PAH-degrading Mycobacterium spp.

引用

页码：700 / 705

页数：6

共 26 条

[1]

[Anonymous], 1983, COLD SPRING HARBOR L

[2] Isolation of adherent polycyclic aromatic hydrocarbon (PAH)-degrading bacteria using PAH-sorbing carriers [J].

Bastiaens, L ;

Springael, D ;

Wattiau, P ;

Harms, H ;

deWachter, R ;

Verachtert, H ;

Diels, L .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (05) :1834-1843

[3] DEGRADATION OF PHENANTHRENE, FLUORENE, FLUORANTHENE, AND PYRENE BY A MYCOBACTERIUM SP [J].

BOLDRIN, B ;

TIEHM, A ;

FRITZSCHE, C .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (06) :1927-1930

[4] Mycobacteria degrading polycyclic aromatic hydrocarbons [J].

Bottger, EC ;

Kirschner, P ;

Springer, B ;

Zumft, W .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1997, 47 (01) :247-247

[5]

Cerniglia Carl E., 1992, Biodegradation, V3, P351, DOI 10.1007/BF00129093

[6]

Churchill SA, 1999, APPL ENVIRON MICROB, V65, P549

[7] Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence (vol 393, pg 537, 1998) [J].

Cole, ST ;

Brosch, R ;

Parkhill, J ;

Garnier, T ;

Churcher, C ;

Harris, D ;

Gordon, SV ;

Eiglmeier, K ;

Gas, S ;

Barry, CE ;

Tekaia, F ;

Badcock, K ;

Basham, D ;

Brown, D ;

Chillingworth, T ;

Conner, R ;

Davies, R ;

Devlin, K ;

Feltwell, T ;

Gentles, S ;

Hamlin, N ;

Holroyd, S ;

Hornsby, T ;

Jagels, K ;

Krogh, A ;

McLean, J ;

Moule, S ;

Murphy, L ;

Oliver, K ;

Osborne, J ;

Quail, MA ;

Rajandream, MA ;

Rogers, J ;

Rutter, S ;

Seeger, K ;

Skelton, J ;

Squares, R ;

Squares, S ;

Sulston, JE ;

Taylor, K ;

Whitehead, S ;

Barrell, BG .

NATURE, 1998, 396 (6707) :190-198

[8] GENE-EXPRESSION IN MYCOBACTERIA - TRANSCRIPTIONAL FUSIONS BASED ON XYLE AND ANALYSIS OF THE PROMOTER REGION OF THE RESPONSE REGULATOR MTRA FROM MYCOBACTERIUM-TUBERCULOSIS [J].

CURCIC, R ;

DHANDAYUTHAPANI, S ;

DERETIC, V .

MOLECULAR MICROBIOLOGY, 1994, 13 (06) :1057-1064

[9] Degradation of pyrene by Mycobacterium flavescens [J].

DeanRoss, D ;

Cerniglia, CE .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1996, 46 (03) :307-312

[10]

Hausner M, 1999, APPL ENVIRON MICROB, V65, P3710

← 1 2 3 →