The Skewness of Alpha Beta T Cell Receptors in Peripheral Blood of the Patients with Type 1 Diabetes

Aim: To detect the skewness of TCR V and TCR V of patients with type 1 diabetes (T1D). Methods: The heparinized venous blood was collected from ten patients with T1D. The peripheral blood lymphocytes (PBL) were isolated and used to extract mRNA. Reverse amplyfication was performed for cDNA synthesis. The skewness of TCR V and V was detected with real-time florescence quantitative polymerase chain reaction (RQ-PCR) and analyzed by DNA melting-curve analysis technique, respectively. Results: Among the TCR V genes, the skewness frequency rate (SFR) of V22 was 30%; both of V5 and V24 were 20%; V28 was the only restricted-clone gene with the SFR of 10%. In all the V genes, V7 and V17 were the the highest expression genes, and their SFRs were both 60%. V11 was near them with the SFR of 40%; the restricted clonal genes were V18 and V20, their SFRs were 10% and 20%, respectivley. Conclusion: There are skewd genes in TCR V and TCR V, which are probably relative to the onset of T1D.