Development and characterization of an enzyme-linked DNA aptamer-magnetic bead-based assay for human IGF-I in serum

被引：16
作者：
Bruno, John G. [1 ]
Richarte, Alicia M. [1 ]
机构：
[1] Operat Technol Corp, San Antonio, TX 78229 USA
来源：
MICROCHEMICAL JOURNAL | 2016年 / 124卷
关键词：
Aptamer; Biomarker; Doping; ELISA; IGF-I; Magnetic bead; GROWTH-FACTOR-I; HORMONE; MARKERS; ABUSE; AXIS;
D O I：
10.1016/j.microc.2015.08.002
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
DNA aptamer sequences were selected and screened for affinity against recombinant human insulin-like growth factor-I (rhIGF-I) using an ELISA-like aptamer-based assay (ELASA). When the top four aptamers were paired against one another in all possible sandwich assay combinations using capture aptamers on magnetic beads (MBs), one sandwich combination appeared superior. This combination produced a reliably sensitive assay with a probable limit of detection <16 ng/mL even in human serum. The assay did not require separation of rhIGF-I from IGF binding proteins (IGFBP) in human serum, suggesting that the capture and reporter aptamers may bind an exposed IGF-I epitope in the IGF-I-IGFBP complex. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：90 / 95
页数：6
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