Dual-color simultaneous structured illumination microscopy based on galvo-mirrors

被引：4
作者：
Yuan, Yifan [1 ]
Liu, Wenjie [1 ,2 ]
Wang, Yueying [1 ]
Yang, Lu [1 ]
Xu, Fanghui [1 ]
Hao, Xiang [1 ]
Han, Yubing [1 ]
Kuang, Cuifang [1 ,2 ,3 ,4 ]
Liu, Xu [1 ]
机构：
[1] Zhejiang Univ, Coll Opt Sci & Engn, State Key Lab Modern Opt Instrumentat, Hangzhou 310027, Peoples R China
[2] Res Ctr Intelligent Chips & Devices, Zhejiang Lab, Hangzhou 311121, Peoples R China
[3] Shanxi Univ, Collaborat Innovat Ctr Extreme Opt, Taiyuan 030006, Peoples R China
[4] Zhejiang Univ, Ningbo Res Inst, Ningbo 315100, Peoples R China
来源：
OPTICS COMMUNICATIONS | 2022年 / 511卷
基金：
中国国家自然科学基金;
关键词：
Fluorescence microscopy; Super-resolution; Live-cell multicolor imaging; Structured illumination; Simultaneous imaging; FLUORESCENCE MICROSCOPY; RESOLUTION LIMIT; CELLS;
D O I：
10.1016/j.optcom.2022.128012
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Structured illumination microscopy (SIM) is one of the most widely used imaging tools in live-cell imaging because of the fast imaging speed and low-intensity requirement. In order to observe the interactions between cell structures, it is necessary to achieve multi-color simultaneous rapid imaging. However, existing systems collect multi-color results in a time-sequential manner. In live-cell imaging, this method brings distortions of the result. In this paper, we provide a dual-color simultaneous SIM system based on galvanometers, which can control the structured pattern of the two-channel flexibly. The resolution capability and simultaneous imaging capability of this system are verified, and the imaging results of living cells are obtained.
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页数：5
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