High-dose ascorbic acid administration improves functional recovery in rats with spinal cord contusion injury

被引：15

作者：

Yan, M. ^{[1
]}

Yang, M. ^{[1
]}

Shao, W. ^{[2
]}

Mao, X-g ^{[3
]}

Yuan, B. ^{[1
]}

Chen, Y-f ^{[1
]}

Ye, Z-x ^{[1
]}

Liang, W. ^{[1
]}

Luo, Z-j ^{[1
]}

机构：

[1] Fourth Mil Med Univ, Xijing Hosp, Dept Orthopaed Surg, Xian 710032, Peoples R China

[2] 359 Hosp Chinese PLA, Dept Orthopaed Surg, Zhenjiang, Jiangsu, Peoples R China

[3] Fourth Mil Med Univ, Xijing Hosp, Dept Neurosurg, Xian 710032, Peoples R China

来源：

SPINAL CORD | 2014年 / 52卷 / 11期

基金：

中国国家自然科学基金;

关键词：

THERAPY;

D O I：

10.1038/sc.2014.135

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Objectives: To evaluate the effects of different doses of ascorbic acid (AA) on the functional performance of rats subjected to standardized spinal cord injury (SCI). Methods: Thirty female Sprague-Dawley rats were divided into three groups (10 animals in each group): control group: rats were subjected to SCI injury and received intraperitoneal saline administration; normal-dose AA group: rats were subjected to SCI injury and received daily intraperitoneal administration of AA at 100 mg kg(-1) bodyweight; high-dose AA group: rats were subjected to SCI injury and received daily intraperitoneal administration of AA at 200 mg kg(-1) bodyweight. The Basso, Beattie, Bresnahan Locomotor Rating Score (BBB score) and footprint analysis were performed to evaluate the functional performance of the rats in each group, and hematoxylin and eosin staining was performed to evaluate necrosis at the injury site. Results: At days 14 and 28 after SCI, rats in the high-dose AA group, but not the normal-dose AA group, exhibited significantly better BBB score compared with the control group (P<0.05). Compared with the control and normal-dose AA group, the high-dose AA group also showed increased stride length, decreased stride width and reduced toe dragging (P<0.05). Histological analysis revealed that both the normal-and high-dose AA groups had reduced necrosis in the injury site compared with the control group (P<0.05). Conclusion: High-dose AA administration during the acute phase post SCI significantly reduced secondary injury-induced tissue necrosis and improved functional performance in rats.

引用

页码：803 / 808

页数：6

共 20 条

[11]

Hewson SM, 2013, EXPERT REV NEUROTHER, V13, P143, DOI [10.1586/ERN.12.157, 10.1586/ern.12.157]

[12] Greatly improved neurological outcome after spinal cord compression injury in AQP4-deficient mice [J].

Saadoun, Samira ;

Bell, B. Anthony ;

Marios, A. S. Verkman ;

Papadopoulos, Marios C. .

BRAIN, 2008, 131 :1087-1098

[13] The effects of medroxy progesterone acetate on the pro-inflammatory cytokines, TNF-alpha and IL-1beta in the early phase of the spinal cord injury [J].

Sahin, Berkant ;

Albayrak, Baki S. ;

Ismailoglu, Ozgur ;

Gorgulu, Askin .

NEUROLOGICAL RESEARCH, 2011, 33 (01) :63-67

[14] From basics to clinical: A comprehensive review on spinal cord injury [J].

Silva, Nuno A. ;

Sousa, Nuno ;

Reis, Rui L. ;

Salgado, Antonio J. .

PROGRESS IN NEUROBIOLOGY, 2014, 114 :25-57

[15] Regeneration beyond the glial scar [J].

Silver, J ;

Miller, JH .

NATURE REVIEWS NEUROSCIENCE, 2004, 5 (02) :146-156

[16] Therapeutic interventions after spinal cord injury [J].

Thuret, Sandrine ;

Moon, Lawrence D. F. ;

Gage, Fred H. .

NATURE REVIEWS NEUROSCIENCE, 2006, 7 (08) :628-643

[17] Spinal Cord Injury: A Review of Current Therapy, Future Treatments, and Basic Science Frontiers [J].

Varma, Abhay K. ;

Das, Arabinda ;

Wallace, Gerald ;

Barry, John ;

Vertegel, Alexey A. ;

Ray, Swapan K. ;

Banik, Naren L. .

NEUROCHEMICAL RESEARCH, 2013, 38 (05) :895-905

[18] The 5th International Symposium of the Nutrition Society Nutritional influences on age-related skeletal muscle loss [J].

Welch, Ailsa A. .

PROCEEDINGS OF THE NUTRITION SOCIETY, 2014, 73 (01) :16-33

[19]

Young W, 2002, PROG BRAIN RES, V137, P231

[20]

Zhang N, 2012, INDIAN J MED RES, V135, P287

← 1 2 →