Self-incompatibility alleles in important genotypes for apple breeding in Brazil

被引:5
作者
Brancher, Thyana Lays [1 ]
Hawerroth, Maraisa Crestani [2 ]
Kvitschal, Marcus Vinicius [2 ]
Manenti, Danielle Caroline [3 ]
Guidolin, Altamir Frederico [4 ]
机构
[1] Univ Fed Lavras, Campus Univ, BR-37200000 Lavras, MG, Brazil
[2] Empresa Pesquisa Agr & Extensao Rural Santa Catar, Estacao Expt Cacador, BR-89501032 Cacador, SC, Brazil
[3] Univ Estadual Maringa, Colombo 5790, BR-87020900 Maringa, Parana, Brazil
[4] Univ Estado Santa Catarina, Ctr Ciencias Agrovet, Luiz de Camoes 2090, BR-88520000 Lages, SC, Brazil
来源
CROP BREEDING AND APPLIED BIOTECHNOLOGY | 2020年 / 20卷 / 04期
关键词
Malus x domestica Borkh; artificial hybridization; S-RNases; S-alleles; RNASE S-ALLELES; IDENTIFICATION; CULTIVARS; GENES; DIVERSITY; CDNA; OLD;
D O I
10.1590/1984-70332020v20n4a54
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The objective of this study was to identify self-incompatibility (S) alleles of advanced breeding selections of apple (Malus x domestica Borkh.). The S-alleles of 42 apple genotypes were analyzed by markers using allele-specific PCR amplification and amplicons digested with restriction endonucleases. Among the screened genotypes were cultivars, advanced selections, and accessions of the Apple Germplasm Bank of Epagri (Cacador, Santa Catarina, Brazil). Two S-alleles were identified in 36 genotypes, and only one S-allele was determined in the other six genotypes. in all, eleven S-alleles were identified among all the genotypes evaluated. The S-3 and S-5 alleles were most frequent (30.2% and 18.6%, respectively). The identification of S-alleles using molecular markers in important apple tree genotypes is useful for determination of compatible parents for breeding programs.
引用
收藏
页码:1 / 9
页数:9
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