Molecular cloning, characterization and mRNA expression of peroxiredoxin in Zhikong scallop Chlamys farreri

被引：26
作者：
Cong, Ming [1 ,2 ]
Ni, Duojiao [1 ,2 ]
Song, Linsheng [1 ]
Wang, Lingling [1 ]
Zhao, Jianmin [1 ]
Qiu, Limei [1 ]
Li, Ling [1 ,2 ]
机构：
[1] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
来源：
MOLECULAR BIOLOGY REPORTS | 2009年 / 36卷 / 06期
关键词：
Chlamys farreri; Fluorescent real-time quantitative PCR; Immune response; Listonella anguillarum; Peroxiredoxin; ANTIOXIDANT ENZYME PEROXIREDOXIN-5; THIOREDOXIN PEROXIDASE; OXIDATIVE STRESS; MYTILUS-EDULIS; NITRIC-OXIDE; HUMAN TENDON; HUMAN LUNG; GENE; APOPTOSIS; FAMILY;
D O I：
10.1007/s11033-008-9335-z
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Peroxiredoxin V (PRX V) is known as an atypical 2-cysteine peroxiredoxin that protects the organisms against various oxidative stresses and functions in signal transduction. The cDNA of a PRX V gene (designated as CfPRX) was cloned from scallop Chlamys farreri. The full-length sequence of CfPRX cDNA was of 2,179 bp with a 564 bp open reading frame encoding a peptide of 187 amino acids. Sequence comparison showed that CfPRX shared higher identities with PRX Vs than that with other isoforms of PRX, indicating CfPRX was a member of the PRX V family. Fluorescent real-time quantitative PCR analysis revealed the presence of CfPRX transcripts in gill filaments, adductor muscle, heart, gonad, kidney and hemocytes, and the stimulation of Listonella anguillarum significantly (P < 0.01) enhanced the mRNA expression of CfPRX in hemocyte. These results indicated that CfPRX was a constitutive and inducible acute-phase protein which was involved in the immune resistance to L. anguillarum stimulation.
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收藏
页码：1451 / 1459
页数：9
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