Flow injection chemiluminescence determination of thiamine based on its enhancing effect on the luminol-hydrogen peroxide system

被引:39
|
作者
Du, JX [1 ]
Li, YH [1 ]
Lu, JR [1 ]
机构
[1] Shaanxi Normal Univ, Dept Chem, Sch Chem & Mat Sci, Xian 710062, Peoples R China
关键词
thiamine; flow injection; chemiluminescence; luminol;
D O I
10.1016/S0039-9140(02)00079-6
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A new flow injection chemiluminescence (CL) method is proposed for the determination of thiamine, based upon its enhancing effect on the CL reaction of luminol with hydrogen peroxide in alkaline solution. The method allows the determination of thiamine within 0.05-8 mug ml(-1) range with a detection limit (3sigma) of 0.01 mug ml(-1). The relative standard deviation is 1.4% (n = 11, 0.5 mug ml(-1) thiamine) and the sample throughput is about 90 samples h(-1). The method was successfully applied to the determination of thiamine in pharmaceutical preparations. (C) 2002 Elsevier Science: B.V. All rights reserved.
引用
收藏
页码:661 / 665
页数:5
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