Label-Free Detection of DNA Polymerase Activity Using Hairpin DNA-Templated Silver Nanoclusters

被引：1
|
作者：
Kim, Seyeon [1 ]
Gang, Jongback [1 ]
机构：
[1] Gachon Univ, Dept Nano Chem, Sungnam 13120, South Korea
来源：
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2019年 / 19卷 / 10期
关键词：
Klenow Fragment; DNA Polymerization; Daidzein; DNA-Templated Silver Nanoclusters (DNA-AgNCs); SENSITIVE DETECTION; COPPER NANOPARTICLES; ASSAY;
D O I：
10.1166/jnn.2019.17073
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Based on the characteristic properties like strong fluorescence and high photostability, DNA-templated silver nanoclusters (DNA-AgNCs) were utilized to detect Klenow fragment exo- (KF-) activity and to monitor its inhibition by daidzein. In this study, fluorescence intensity is linearly correlated for KF-concentration ranging from 0 to 2.0 U/mL, with a minimum detectable concentration of 0.05 U/mL. In addition, KF- inhibition was monitored by changes in fluorescence intensity with increasing daidzein concentration gradually.
引用
收藏
页码：6534 / 6538
页数：5
相关论文
共 50 条
  • [1] A Label-Free Detection of NdeI Endonuclease Activity by Using DNA-Templated Silver Nanoclusters
    Lee, Chunghyun
    Gang, Jongback
    JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 2018, 18 (09) : 6339 - 6342
  • [2] RNase A activity analysis and imaging using label-free DNA-templated silver nanoclusters
    Dang, Wenya
    Luo, Ruxin
    Fan, Jialong
    Long, Ying
    Tong, Chunyi
    Xiao, Feng
    Xiong, Xiang
    Liu, Bin
    TALANTA, 2020, 209
  • [3] The detection of a mismatched DNA by using hairpin DNA-templated silver nanoclusters
    Kim, Seyeon
    Gang, Jongback
    ANALYTICAL BIOCHEMISTRY, 2018, 549 : 171 - 173
  • [4] DNA-templated silver nanoclusters as label-free fluorescent probes for detection of bleomycin
    Chang, Yong
    Zhang, Pu
    Yu, Yan
    Du, Yu Qing
    Wang, Wei
    Huang, Cheng Zhi
    ANALYTICAL METHODS, 2013, 5 (21) : 6200 - 6204
  • [5] Label-free probes using DNA-templated silver nanoclusters as versatile reporters
    Guo, Yahui
    Pan, Xinyue
    Zhang, Wenya
    Hu, Zhigang
    Wong, Ka-Wang
    He, Zhike
    Li, Hung-Wing
    BIOSENSORS & BIOELECTRONICS, 2020, 150 (150):
  • [6] A label-free fluorescence method for actin detection based on DNA-templated silver nanoclusters
    Chen, Mingjian
    Ma, Changbei
    Yan, Ying
    ANALYTICAL METHODS, 2019, 11 (34) : 4348 - 4353
  • [7] Label-free fluorescent detection of copper(II) using DNA-templated highly luminescent silver nanoclusters
    Zhang, Min
    Ye, Bang-Ce
    ANALYST, 2011, 136 (24) : 5139 - 5142
  • [8] Label-Free Rapid and Simple Detection of Exonuclease III Activity with DNA-Templated Copper Nanoclusters
    Lee, Chunghyun
    Gang, Jongback
    JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2018, 28 (09) : 1467 - 1472
  • [9] A label-free aptasensor for the detection of ATP based on turn-on fluorescence DNA-templated silver nanoclusters
    Zhang, Baozhu
    Yang, Ziyao
    Li, Yuxia
    Ma, Ling
    Li, Fenfang
    Lv, Xiuqing
    Wen, Guangming
    RSC ADVANCES, 2022, 12 (46) : 30024 - 30029
  • [10] DNA-Templated Silver Nanoclusters Used as a Label-Free Fluorescent Probe for the Detection of O6-Methyltransferase Activity
    Van-Trong Nguyen
    Tran, T. T. Nha
    Thanh-Khue Van
    ThanhThuy Tran
    JOURNAL OF ANALYTICAL CHEMISTRY, 2021, 76 (05) : 585 - 591
12345