Shear stress-induced shedding of soluble intercellular adhesion molecule-1 from saphenous vein endothelium

被引:39
作者
Sultan, S
Gosling, M
Nagase, H
Powell, JT
机构
[1] Univ London Imperial Coll Sci & Technol, Dept Vasc Surg, London W6 8RP, England
[2] Univ London Imperial Coll Sci & Technol, Kennedy Inst, Rheumatol Div, London W6 8LH, England
基金
英国惠康基金;
关键词
endothelium; shear stress; adhesion molecule; metalloprotemase;
D O I
10.1016/S0014-5793(04)00337-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Within 6 h, shear stress upregulated intercellular adhesion molecule-1 (ICAM-1) (two- to four-fold, P < 0.001) and induced matrix metalloproteinase-2 (MMP-2) in cultured human saphenous vein endothelial cells. By 8 h endothelial ICAM-1 levels returned to baseline, with concomitant increase in soluble ICAM-1 (sICAM-1) (P < 0.001) and MMP-9 had been induced. Inclusion of a hydroxamate metalloproteinase inhibitor partially reversed the effects on ICAM-1 and sICAM-1 at 8 h, whereas TIMP-1, -2 or -3 had no effect. MMP-9, but not MMP-2, co-immunoprecipitated with ICAM-1. sICAM-1 was processed distal to Arg441, indicating that MMP-9, docking to ICAM-1, contributes to sICAM-1 shedding and attenuation of the shear stress-induced upregulation of ICAM-1. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:161 / 165
页数:5
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