POTATO LEAF EXTRACT AND ITS COMPONENT, α-SOLANINE, EXERT SIMILAR IMPACTS ON DEVELOPMENT AND OXIDATIVE STRESS IN Galleria mellonella L.

被引：25

作者：

Adamski, Zbigniew ^{[1
,2
]}

Marciniak, Pawel ^{[2
]}

Ziemnicki, Kazimierz ^{[2
]}

Buyukgzel, Ender ^{[3
]}

Erdem, Meltem ^{[4
]}

Buyukguzel, Kemal ^{[5
]}

Ventrella, Emanuela ^{[6
]}

Falabella, Patrizia ^{[6
]}

Cristallo, Massimo ^{[6
]}

Salvia, Rosanna ^{[6
]}

Bufo, Sabino Aurelio ^{[6
]}

Scrano, Laura ^{[7
]}

机构：

[1] Adam Mickiewicz Univ, Fac Biol, Elect & Confocal Microscope Lab, Poznan, Poland

[2] Adam Mickiewicz Univ, Fac Biol, Dept Anim Physiol & Dev Biol, Poznan, Poland

[3] Bulent Ecevit Univ, Fac Arts & Sci, Dept Mol Biol & Genet, Zonguldak, Turkey

[4] Bulent Ecevit Univ, Ahmet Erdooan Vocat Sch Hlth Serv, Zonguldak, Turkey

[5] Bulent Ecevit Univ, Fac Arts & Sci, Dept Biol, Zonguldak, Turkey

[6] Univ Basilicata, Dipartimento Sci, I-85100 Potenza, Italy

[7] Univ Basilicata, Dept European & Mediterranean Cultures, Matera, Italy

来源：

ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY | 2014年 / 87卷 / 01期

关键词：

potato leaf extract; glycoalkaloids; Galleria mellonella; development; oxidative stress; GST; ANTIOXIDANT ENZYMES; TOMATO GLYCOALKALOIDS; PLANT; DEFENSE; DETOXIFICATION; TRANSFERASES; PERFORMANCE; TOXICITY; APHID; GUT;

D O I：

10.1002/arch.21177

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plants synthesize a broad range of secondary metabolites that act as natural defenses against plant pathogens and herbivores. Among these, potato plants produce glycoalkaloids (GAs). In this study, we analyzed the effects of the dried extract of fresh potato leaves (EPL) on the biological parameters of the lepidopteran, Galleria mellonella (L.) and compared its activity to one of the main EPL components, the GA alpha-solanine. Wax moth larvae were reared from first instar on a diet supplemented with three concentrations of EPL or alpha-solanine. Both EPL and alpha-solanine affected survivorship, fecundity, and fertility of G. mellonella to approximately the same extent. We evaluated the effect of EPL and alpha-solanine on oxidative stress in midgut and fat body by measuring malondialdehyde (MDA) and protein carbonyl (PCO) contents, both biomarkers of oxidative damage. We evaluated glutathione S-transferase (GST) activity, a detoxifying enzyme acting in prevention of oxidative damage. EPL and alpha-solanine altered MDA and PCO concentrations and GST activity in fat body and midgut. We infer that the influence of EPL on G. mellonella is not enhanced by synergistic effects of the totality of potato leaf components compared to alpha-solanine alone. (C) 2014 Wiley Periodicals, Inc.

引用

页码：26 / 39

页数：14

共 42 条

[1] Effect of Various Xenobiotics on Hatching Success of Spodoptera exigua Eggs as Compared to a Natural Plant Extract [J].