Effect of Urban Wastewater Discharge on the Abundance of Antibiotic Resistance Genes and Antibiotic-Resistant Escherichia coli in Two Italian Rivers

被引:15
|
作者
Pantanella, Fabrizio [1 ]
Lekunberri, Itziar [2 ]
Gagliardi, Antonella [1 ]
Venuto, Giuseppe [1 ]
Sanchez-Melsio, Alexandre [2 ]
Fabiani, Massimo [1 ]
Balcazar, Jose Luis [2 ]
Schippa, Serena [1 ]
De Giusti, Maria [1 ]
Borrego, Cartes [2 ,3 ]
Solimini, Angelo [1 ]
机构
[1] Univ Roma La Sapienza, Dept Publ Hlth & Infect Dis, Piazza A Moro 5, I-00185 Rome, Italy
[2] Univ Girona, Catalan Inst Water Res ICRA, Sci & Technol Pk, Girona 17003, Spain
[3] Univ Girona, Inst Aquat Ecol, Grp Mol Microbial Ecol, Girona 17001, Spain
关键词
antibiotic resistance; antibiotic resistance genes; Escherichia coli; Arrone River; Tiber River; 1; INTEGRON-INTEGRASE; TREATMENT PLANTS; ANTHROPOGENIC PRESSURE; ONE HEALTH; IMPACT; PHARMACEUTICALS; ENVIRONMENT; BACTERIA;
D O I
10.3390/ijerph17186813
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Background: Wastewater treatment plants (WWTPs) are microbial factories aimed to reduce the amount of nutrients and pathogenic microorganisms in the treated wastewater before its discharge into the environment. We studied the impact of urban WWTP effluents on the abundance of antibiotic resistance genes (ARGs) and antibiotic-resistant Escherichia coli (AR-E. coli) in the last stretch of two rivers (Arrone and Tiber) in Central Italy that differ in size and flow volume. Methods: Water samples were collected in three seasons upstream and downstream of the WWTP, at the WWTP outlet, and at sea sites near the river mouth, and analyzed for the abundance of ARGs by qPCR and AR-E. coli using cultivation followed by disk diffusion assays. Results: For all studied genes (16S rRNA, intI1, sul1, ermB, blaTEM, tetW and qnrS), absolute concentrations were significantly higher in the Tiber than in the Arrone at all sampling sites, despite their collection date, but the prevalence of target ARGs within bacterial communities in both rivers was similar. The absolute concentrations of most ARGs were also generally higher in the WWTP effluent with median levels between log 4 and log 6 copies per ml but did not show differences along the studied stretches of rivers. Statistically significant site effect was found for E. coli phenotypic resistance to tetracycline and ciprofloxacin in the Arrone but not in the Tiber. Conclusions: In both rivers, diffuse or point pollution sources other than the studied WWTP effluents may account for the observed resistance pattern, although the Arrone appears as more sensitive to the wastewater impact considering its lower flow volume.
引用
收藏
页码:1 / 13
页数:13
相关论文
共 50 条
  • [41] Antibiotic-resistant bacteria, antibiotic resistance genes, and antibiotic residues in wastewater from a poultry slaughterhouse after conventional and advanced treatments
    Savin, Mykhailo
    Alexander, Johannes
    Bierbaum, Gabriele
    Hammerl, Jens Andre
    Hembach, Norman
    Schwartz, Thomas
    Schmithausen, Ricarda Maria
    Sib, Esther
    Voigt, Alexander
    Kreyenschmidt, Judith
    SCIENTIFIC REPORTS, 2021, 11 (01)
  • [42] Antibiotic-resistant bacteria, antibiotic resistance genes, and antibiotic residues in wastewater from a poultry slaughterhouse after conventional and advanced treatments
    Mykhailo Savin
    Johannes Alexander
    Gabriele Bierbaum
    Jens Andre Hammerl
    Norman Hembach
    Thomas Schwartz
    Ricarda Maria Schmithausen
    Esther Sib
    Alexander Voigt
    Judith Kreyenschmidt
    Scientific Reports, 11
  • [43] A global perspective on the abundance, diversity and mobility of antibiotic resistance genes in Escherichia coli
    Qing, Yun
    Zou, Zhongai
    Jiang, Guolian
    Qin, Lingshi
    Liu, Kehui
    Liu, Zongbao
    FRONTIERS IN VETERINARY SCIENCE, 2024, 11
  • [44] The Fate and Occurrence of Antibiotic-Resistant Bacteria and Antibiotic Resistance Genes during Advanced Wastewater Treatment and Disinfection: A Review
    Kalli, Maria
    Noutsopoulos, Constantinos
    Mamais, Daniel
    WATER, 2023, 15 (11)
  • [45] Inactivation of antibiotic-resistant bacteria and antibiotic-resistance genes in wastewater streams: Current challenges and future perspectives
    Mosaka, Thabang B. M.
    Unuofin, John O.
    Daramola, Michael O.
    Tizaoui, Chedly
    Iwarere, Samuel A.
    FRONTIERS IN MICROBIOLOGY, 2023, 13
  • [46] The occurrence of disinfectant and antibiotic-resistant genes in Escherichia coli isolated from chickens in Egypt
    Ibrahim, Waleed A.
    Marouf, Sherif A.
    Erfan, Ahmed M.
    Nasef, Soad A.
    El Jakee, Jakeen K.
    VETERINARY WORLD, 2019, 12 (01) : 141 - 145
  • [47] Effect of photocatalysis on the transfer of antibiotic resistance genes in urban wastewater
    Dunlop, P. S. M.
    Ciavola, M.
    Rizzo, L.
    McDowell, D. A.
    Byrne, J. A.
    CATALYSIS TODAY, 2015, 240 : 55 - 60
  • [48] Effects of hydrothermal treatment on the reduction of antibiotic-resistant Escherichia coli and antibiotic resistance genes and the fertilizer potential of liquid product from cattle manure
    Im, Dongbeom
    Chen, Yiren
    Nishimura, Fumitake
    WASTE MANAGEMENT, 2024, 183 : 123 - 131
  • [49] Removal of antibiotic resistance genes and inactivation of antibiotic-resistant bacteria by oxidative treatments
    Zhao, Xiaoyu
    Su, Haochang
    Xu, Wujie
    Hu, Xiaojuan
    Xu, Yu
    Wen, Guoliang
    Cao, Yucheng
    SCIENCE OF THE TOTAL ENVIRONMENT, 2021, 778
  • [50] Survey on tetracycline resistance and antibiotic-resistant genotype of avian Escherichia coli in North China
    Zhang, T.
    Wang, C. G.
    Lv, J. C.
    Wang, R. S.
    Zhong, X. H.
    POULTRY SCIENCE, 2012, 91 (11) : 2774 - 2777