Effect of Urban Wastewater Discharge on the Abundance of Antibiotic Resistance Genes and Antibiotic-Resistant Escherichia coli in Two Italian Rivers

被引:15
作者
Pantanella, Fabrizio [1 ]
Lekunberri, Itziar [2 ]
Gagliardi, Antonella [1 ]
Venuto, Giuseppe [1 ]
Sanchez-Melsio, Alexandre [2 ]
Fabiani, Massimo [1 ]
Balcazar, Jose Luis [2 ]
Schippa, Serena [1 ]
De Giusti, Maria [1 ]
Borrego, Cartes [2 ,3 ]
Solimini, Angelo [1 ]
机构
[1] Univ Roma La Sapienza, Dept Publ Hlth & Infect Dis, Piazza A Moro 5, I-00185 Rome, Italy
[2] Univ Girona, Catalan Inst Water Res ICRA, Sci & Technol Pk, Girona 17003, Spain
[3] Univ Girona, Inst Aquat Ecol, Grp Mol Microbial Ecol, Girona 17001, Spain
关键词
antibiotic resistance; antibiotic resistance genes; Escherichia coli; Arrone River; Tiber River; 1; INTEGRON-INTEGRASE; TREATMENT PLANTS; ANTHROPOGENIC PRESSURE; ONE HEALTH; IMPACT; PHARMACEUTICALS; ENVIRONMENT; BACTERIA;
D O I
10.3390/ijerph17186813
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Background: Wastewater treatment plants (WWTPs) are microbial factories aimed to reduce the amount of nutrients and pathogenic microorganisms in the treated wastewater before its discharge into the environment. We studied the impact of urban WWTP effluents on the abundance of antibiotic resistance genes (ARGs) and antibiotic-resistant Escherichia coli (AR-E. coli) in the last stretch of two rivers (Arrone and Tiber) in Central Italy that differ in size and flow volume. Methods: Water samples were collected in three seasons upstream and downstream of the WWTP, at the WWTP outlet, and at sea sites near the river mouth, and analyzed for the abundance of ARGs by qPCR and AR-E. coli using cultivation followed by disk diffusion assays. Results: For all studied genes (16S rRNA, intI1, sul1, ermB, blaTEM, tetW and qnrS), absolute concentrations were significantly higher in the Tiber than in the Arrone at all sampling sites, despite their collection date, but the prevalence of target ARGs within bacterial communities in both rivers was similar. The absolute concentrations of most ARGs were also generally higher in the WWTP effluent with median levels between log 4 and log 6 copies per ml but did not show differences along the studied stretches of rivers. Statistically significant site effect was found for E. coli phenotypic resistance to tetracycline and ciprofloxacin in the Arrone but not in the Tiber. Conclusions: In both rivers, diffuse or point pollution sources other than the studied WWTP effluents may account for the observed resistance pattern, although the Arrone appears as more sensitive to the wastewater impact considering its lower flow volume.
引用
收藏
页码:1 / 13
页数:13
相关论文
共 50 条
  • [31] Removal of antibiotic resistance genes and inactivation of antibiotic-resistant bacteria by oxidative treatments
    Zhao, Xiaoyu
    Su, Haochang
    Xu, Wujie
    Hu, Xiaojuan
    Xu, Yu
    Wen, Guoliang
    Cao, Yucheng
    SCIENCE OF THE TOTAL ENVIRONMENT, 2021, 778
  • [32] Impact of Manure Fertilization on the Abundance of Antibiotic-Resistant Bacteria and Frequency of Detection of Antibiotic Resistance Genes in Soil and on Vegetables at Harvest
    Marti, Romain
    Scott, Andrew
    Tien, Yuan-Ching
    Murray, Roger
    Sabourin, Lyne
    Zhang, Yun
    Topp, Edward
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2013, 79 (18) : 5701 - 5709
  • [33] Advanced treatment of urban wastewater by UV radiation: Effect on antibiotics and antibiotic-resistant E. coli strains
    Rizzo, Luigi
    Fiorentino, Antonino
    Anselmo, Antonella
    CHEMOSPHERE, 2013, 92 (02) : 171 - 176
  • [34] Occurrence of multidrug-resistant Escherichia coli and antibiotic resistance genes in a wastewater treatment plant and its associated river water in Harare, Zimbabwe
    Takawira, Hilary
    Mbanga, Joshua
    WATER SA, 2023, 49 (04) : 396 - 403
  • [35] Transmission of Antibiotic-Resistant Escherichia coli from Chicken Litter to Agricultural Soil
    Fatoba, Dorcas Oladayo
    Amoako, Daniel Gyamfi
    Abia, Akebe Luther King
    Essack, Sabiha Yusuf
    FRONTIERS IN ENVIRONMENTAL SCIENCE, 2022, 9
  • [36] Effect of poultry litter soil amendment on antibiotic-resistant Escherichia coli
    Agga, Getahun E.
    Durso, Lisa M.
    Sistani, Karamat R.
    JOURNAL OF ENVIRONMENTAL QUALITY, 2024, 53 (03) : 300 - 313
  • [37] Transport of antibiotic-resistant Escherichia coli in a public rural karst water supply
    Laroche, Emilie
    Petit, Fabienne
    Fournier, Matthieu
    Pawlak, Barbara
    JOURNAL OF HYDROLOGY, 2010, 392 (1-2) : 12 - 21
  • [38] Human exposure assessment to antibiotic-resistant Escherichia coli through drinking water
    O'Flaherty, E.
    Borrego, C. M.
    Balcazar, J. L.
    Cummins, E.
    SCIENCE OF THE TOTAL ENVIRONMENT, 2018, 616 : 1356 - 1364
  • [39] Reusing Treated Wastewater: Consideration of the Safety Aspects Associated with Antibiotic-Resistant Bacteria and Antibiotic Resistance Genes
    Hong, Pei-Ying
    Julian, Timothy R.
    Pype, Marie-Laure
    Jiang, Sunny C.
    Nelson, Kara L.
    Graham, David
    Pruden, Amy
    Manaia, Celia M.
    WATER, 2018, 10 (03)
  • [40] Performance evaluation of ozonation for removal of antibiotic-resistant Escherichia coli and Pseudomonas aeruginosa and genes from hospital wastewater
    Asghari, Farzaneh Baghal
    Dehghani, Mohammad Hadi
    Dehghanzadeh, Reza
    Farajzadeh, Davoud
    Shanehbandi, Dariush
    Mahvi, Amir Hossein
    Yaghmaeian, Kamyar
    Rajabi, Akbar
    SCIENTIFIC REPORTS, 2021, 11 (01)