Agarolytic bacterium Persicobacter sp CCB-QB2 exhibited a diauxic growth involving galactose utilization pathway

被引：9
作者：
Furusawa, Go [1 ]
Lau, Nyok-Sean [1 ]
Suganthi, Appalasamy [1 ,2 ]
Amirul, Abdullah Al-Ashraf [1 ,3 ]
机构：
[1] Univ Sains Malaysia, Ctr Chem Biol, Bayan Lepas, Malaysia
[2] Univ Malaysia Kelantan, Fac Earth Sci, Jeli, Malaysia
[3] Univ Sains Malaysia, Sch Biol Sci, Minden, Malaysia
来源：
MICROBIOLOGYOPEN | 2017年 / 6卷 / 01期
关键词：
agarase; agarolytic bacterium; diauxic growth; leloir pathway; Persicobacter; CARBOHYDRATE-BINDING MODULES; AGARIVORANS SP HZ105; SP STRAIN JT0107; BETA-AGARASE; MARINE BACTERIUM; EXTRACELLULAR AGARASE; CRYSTAL-STRUCTURE; SP-NOV; BIOCHEMICAL-CHARACTERIZATION; PSEUDOMONAS-ATLANTICA;
D O I：
10.1002/mbo3.405
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The agarolytic bacterium Persicobacter sp. CCB-QB2 was isolated from seaweed (genus Ulva) collected from a coastal area of Malaysia. Here, we report a high-quality draft genome sequence for QB2. The Rapid Annotation using Subsystem Technology (RAST) annotation server identified four -agarases (PdAgaA, PdAgaB, PdAgaC, and PdAgaD) as well as galK, galE, and phosphoglucomutase, which are related to the Leloir pathway. Interestingly, QB2 exhibited a diauxic growth in the presence of two kinds of nutrients, such as tryptone and agar. In cells grown with agar, the profiles of agarase activity and growth rate were very similar. galK, galE, and phosphoglucomutase genes were highly expressed in the second growth phase of diauxic growth, indicating that QB2 cells use galactose hydrolyzed from agar by its agarases and exhibit nutrient prioritization. This is the first report describing diauxic growth for agarolytic bacteria. QB2 is a potential novel model organism for studying diauxic growth in environmental bacteria.
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页数：11
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